摘要
目的 探究慢性1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导帕金森病(PD)模型小鼠黑质(SN)和外侧苍白球(GPe)脑区星形胶质细胞、小胶质细胞数目及铁沉积的变化。方法 将健康雄性8周龄C57BL/6小鼠随机分成对照组和MPTP组,MPTP组小鼠腹腔注射MPTP(18 mg/kg体质量),对照组给予等体积生理盐水,每周2次,持续5周。通过爬杆实验检测小鼠运动功能,组织免疫荧光染色检测酪氨酸羟化酶(TH)神经元、星形胶质细胞和小胶质细胞数目,普鲁士蓝铁染色(Perl’s-DAB)检测铁沉积。结果 与对照组相比,MPTP组小鼠爬杆转头时间明显增加,差异有统计学意义(t=2.42,P<0.05);SN中TH神经元数目减少,星形胶质细胞和小胶质细胞数目增加,铁阳性细胞数增加,差异均有统计学意义(t=3.82~4.83,P<0.05);GPe中小胶质细胞数目显著增多(t=2.54,P<0.05),星形胶质细胞和铁阳性细胞数目无变化。结论 MPTP诱导的PD小鼠模型建模成功。模型鼠SN胶质细胞激活和铁沉积均增多,而GPe中仅有小胶质细胞激活增多。
Objective To investigate the changes inastrocytes,microglial cells,and iron deposition in the substantia nigra(SN)and the externalglobus pallidus(GPe)in a mouse model of Parkinson’s disease(PD)induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Healthy male C57BL/6 mice,aged 8 weeks,were randomly divided into control group and MPTP group.The mice in the MPTP group were intraperitoneally injected with MPTP at a dose of 18 mg/kg body weight,while those in the control group were given an equal volume of normal saline,twice a week for 5 weeks.The pole test was used to measure the motor function of mice;immunofluorescent staining was used to measure the numbers of tyrosine hydroxylase(TH)neurons,astrocytes,and microglial cells;Prussian blue iron staining was used to observe iron deposition.Results Compared with the control group,the MPTP group had a significant increase in the time to turn around(t=2.42,P<0.05),a significant reduction in the number of TH neurons and significant increases in the numbers of astrocytes,microglial cells,and iron-positive cells in the SN(t=3.82-4.83,P<0.05),and a significant increase in the number of microglial cells in the GPe(t=2.54,P<0.05),but there were no significant changes in the numbers of astrocytes and iron-positive cells in the GPe.Conclusion The mouse model of PD induced by MPTP is successfully established.There are increases in glial cell activation and iron deposition in the SN of model mice,while there is only an increase in microglial cell activation in the GPe.
作者
李梦雨
马希珍
谢俊霞
宋宁
LI Mengyu;MA Xizhen;XIE Junxia;SONG Ning(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)
出处
《青岛大学学报（医学版）》
CAS
2024年第3期350-354,共5页
Journal of Qingdao University(Medical Sciences)
基金
国家自然科学基金面上项目(31871049)。
