摘要
为探究猕猴桃花粉萌发活力和花粉萌发前后的形态变化,以15份美味猕猴桃花粉为样本,采用离体培养方法测定花粉的萌发活力,利用扫描电镜观察离体培养前后5份雌株花粉和10份雄株花粉的形态变化。结果表明:15份花粉样本萌发率差异较大,5份雌株花粉均不萌发;10份雄株花粉中8号花粉样本的萌发率最高,为79.27%;9号花粉样本的萌发率最低,仅为22.85%。15份花粉样本离体培养前后扫描电镜观察发现:极面观基本相同,均为三裂圆形;赤道面观有差异,5份雌株花粉离体培养前后花粉形态不发生改变,均为近球形,而10份雄株花粉在萌发前为超长球形或长球形,萌发后均为近球形,极轴长度略有变小、赤道轴长变大、极轴长度与赤道轴长度比值变小、萌发沟宽度增宽,但萌发后的雄株花粉样本间各项形态学指标值差异较小。
In order to investigate the viability of kiwifruit pollen,and to study the morphological changes before and after pollen germination,15 samples of Actinidia deliciosa pollens were used in this experiment.An in vitro culture method was used to determine pollen germination viability,and scanning electron microscopy was used to observe the morphology of five female pollens and ten male pollens before and after germination.The results showed that the germination rate of pollens from male plants varied considerably.None of the five female pollens germinated.Among the 10 male pollen samples No.8 had the highest germination rate of 79.27%and No.9 had the lowest germination rate of 22.85%.The polar facial view of the 15 samples did not change before and after ex vivo incubation.These samples were all trilobate circles.However,there was a significant difference in their equatorial view.The pollen morphology did not change before and after the female plants were cultured in isolation,and they were all spheroidal.The pollens of the male plant were perprolate or prolate before germination,and after germination in culture in vitro,all 10 samples were sub-spherical,with the polar axis length becoming almost smaller,the equatorial axis length becoming larger,the P/E ratio becoming smaller,and the width of the germination groove becoming larger.The differences in the values of the morphological indexes between the samples were small.
作者
何利钦
王丽华
郑晓琴
张茜
梁爽
李峤虹
HE Liqin;WANG Lihua;ZHENG Xiaoqin;ZHANG Qian;LIANG Shuang;LI Qiaohong(Sichuan Provincial Academy of Natural Resource Sciences,Chengdu 610015,China;China-New Zealand Belt and Road Joint Laboratory on Kiwifruit,Chengdu 610015,China;College of Food and Bioengineering,Chengdu University,Chengdu 610106,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2024年第4期78-82,共5页
Journal of Biology
基金
四川省科技计划项目(2021YFYZ0023,2021YFN0039)
四川省中央引导地方科技发展专项项目(2022ZYD0123)
四川省省级科研院所基本科研业务费项目(2021JDKY0023)。
关键词
猕猴桃
花粉
离体培养
萌发活力
形态学
kiwifruit
pollen
in vitro incubation
germination vigor
morphology
