摘要
目的观察一种双链季铵盐消毒液在-40℃低温环境中的消毒效果,为低温消毒应用提供参考。方法采用载体定量杀菌试验和现场消毒试验方法,对该双链季铵盐消毒液在低温条件下环境表面消毒效果进行观察。结果该双链季铵盐消毒液原液含量为37 g/L,pH值为5.03。该消毒液在-40℃下放置>8 h后无析出,无结晶。在-40℃低温冷库中,原液喷洒作用30 min,对织物样片上大肠埃希菌和金黄色葡萄球菌的杀灭对数值均>3.00;原液按10 mL/m^(3)喷雾作用60 min,对玻璃表面的大肠埃希菌和金黄色葡萄球菌杀灭对数值均>3.00。该消毒液原液对新西兰兔完整皮肤无刺激性,对小鼠急性经口毒性试验为无毒级,对小鼠无致微核作用。结论该双链季铵盐消毒液在-40℃低温下可杀灭环境表面大肠埃希菌和金黄色葡萄球菌,属于实际无毒物质。
Objective To observe the disinfection effect of a double-chain quaternary ammonium salt disinfectant in a low-temperature environment of-40℃,and to provide reference for the application of low-temperature disinfection.Methods Carrier quantitative bactericidal test and on-site disinfection test methods were used to observe the disinfection effect of the double-chain quaternary ammonium salt disinfectant on environmental surfaces under low temperature conditions.Results The content of stock solution of the double-chain quaternary ammonium salt disinfectant was 37 g/L,and the pH value was 5.03.This disinfectant has no precipitation or crystallization after being placed at-40℃for>8 h.In a low-temperature cold storage at-40℃,the stock solution was sprayed for 30 min,and the killing log values of Escherichia coli and Staphylococcus aureus on the fabric samples were both>3.00;the stock solution was sprayed at 10 mL/m^(3)for 60 min,and the killing log values of Escherichia coli and Staphylococcus aureus on the glass surface were both>3.00.The stock solution of the disinfectant was non-irritating to the intact skin of New Zealand rabbits.The acute oral toxicity test on mice was non-toxic and had no micronucleogenic effect on mice.Conclusion This double-chain quaternary ammonium salt disinfectant can kill Escherichia coli and Staphylococcus aureus on environmental surfaces at low temperature of-40℃,and is an actual non-toxic substance.
作者
钟秋彤
张显勇
谭俊杰
邱智华
何威
叶俊豪
ZHONG Qiu-tong;ZHANG Xian-yong;TAN Jun-jie;QIU Zhi-hua;HE Wei;YE Jun-hao(Guangzhou Institute of Microbiology Co.,Ltd.,Guangzhou Guangdong 510663,China)
出处
《中国消毒学杂志》
CAS
2024年第7期489-491,共3页
Chinese Journal of Disinfection
关键词
双链季铵盐
低温消毒剂
物体表面
消毒效果
double-chain quaternary ammonium salt
low-temperature disinfectant
object surface
disinfection effect
