摘要
干旱严重影响作物的生长和产量,根系的结构及分布情况是植物响应干旱胁迫的关键因素。高粱作为一种耐旱性较强的作物,挖掘其抗旱相关基因对于作物抗旱改良具有重要的作用。本研究在高粱中克隆得到深根基因SbDRO1,对其进行生物信息学分析,并在拟南芥中进行功能验证。结果表明,SbDRO1基因CDS全长759 bp,编码252个氨基酸;在拟南芥中过表达SbDRO1能够显著提高拟南芥在干旱条件下的存活率,且转基因拟南芥的根系相对野生型明显发达。进一步研究发现,涉及生长素合成、极性运输以及抗逆相关基因的表达量在转基因拟南芥中显著提高,这或许是SbDRO1基因提高转基因拟南芥抗旱性的主要分子机理。本研究结果可为农作物抗旱改良提供潜在的基因资源。
Drought has seriously affected growth and yield of crops.The structure and distribution of roots were key factors in plant response to drought stress.As a crop with strong drought resistance,exploring drought resistance related genes in sorghum plays an important role in improving crop drought resistance.In this study,the deep rooting gene SbDRO1 was cloned from sorghum,and then its bioinformaticsan alysis was conducted,and its function was validated in Arabidopsis.The results indicated that the total length of CDS of SbDRO1 gene was 759 bp encoding 252 amino acids.Overexpression of SbDRO1 gene could significantly improve Arabidopsis survival rate under drought conditions,and the root system of transgenic Arabidopsis was significantly developed compared to the wild type.Further research found that the expression of genes related to auxin synthesis,polar auxin transport and stress resistance in transgenic Arabidopsis was significantly higher than that in wild type,which might be the main molecular mechanism of SbDRO1 gene improving drought resistance of transgenic Arabidopsis.These results could provide potential genetic resources for crop drought resistance improvement.
作者
曾廷儒
程文
孙琦
岳润清
李文兰
张华
高日新
张茂林
Zeng Tingru;Cheng Wen;Sun Qi;Yue Runqing;Li Wenlan;Zhang Hua;Gao Rixin;Zhang Maolin(Maize Research Institute,Shandong Academy of Agricultural Sciences/National Engineering Research Center for Wheat and Maize,Jinan 250100,China)
出处
《山东农业科学》
北大核心
2024年第7期23-31,共9页
Shandong Agricultural Sciences
基金
山东省农业良种工程项目(2022LZGC019)
山东省自然科学基金面上项目(ZR2020MC111,ZR2021MC084)。
