摘要
针对部分油气田服务企业以平板计数法、ATP荧光快速检测法代替绝迹稀释法(MPN)进行页岩气生产液中细菌含量检测的现象,将这三种细菌计数方法对相同样品的测定结果进行了对比研究。平板计数法与ATP荧光法操作均比MPN法简便,但是对于页岩气生产液,这两种方法均存在检测出的细菌类型不明确、计数结果不准确、线性关系差等缺点。平板计数法检测出的细菌主要为好氧或耐氧菌腐生菌,无法检测和鉴别与腐蚀密切相关的硫酸盐还原菌。ATP荧光法通过总菌的ATP含量换算得到细菌含量,该法目前更多用于医疗与食品行业的限制类细菌含量的快速检测,尚无明确标准可依,且无法区分菌体的死活。而MPN法可通过鉴别培养基将与腐蚀相关的硫酸盐还原菌(SRB)、铁细菌(IB)和腐生菌(TGB)分别培养后计数,且其检测限远低于另外两种方法,因此更适用于页岩气生产液中特定腐蚀菌种的检测。
In view of the phenomenon that some oil and gas field service enterprises use plate counting method and ATP fluorescence rapid detection method instead of most probable number(MPN)to detect the bacterial content in shale gas production fluid,a comparative study was conducted on the determined results of the same sample using these three bacterial counting methods.The operation of plate counting method and the ATP fluorescence method is simpler than that of MPN method,but for shale gas production fluid,these two methods have the disadvantages of unclear detected bacterial types,inaccurate counting results and poor linear relationship.The bacteria detected by the plate counting method are mainly aerobic or oxygen-resistant saprophytic bacteria,and it is impossible to detect and identify sulfate-reducing bacteria closely related to corrosion.The ATP fluorescence method converts the total bacterial ATP content to obtain bacterial content.Currently,this method is more commonly used for rapid detection of restricted bacterial content in the medical and food industries,and there is no clear standard to rely on,and it cannot distinguish between dead and alive bacterial bodies.The MPN method can count the sulfate reducing bacteria(SRB),iron bacteria(IB)and saprophytic bacteria(TGB)related to corrosion by identifying the culture medium,and its detection limit is much lower than the other two methods.Therefore,it is more suitable for the detection of specific corrosion bacteria in shale gas production fluid.
作者
陈建强
薛松松
欧天雄
徐波
邱海燕
Chen Jianqiang;Xue Songsong;Ou Tianxiong;Xu Bo;Qiu Haiyan(Southwest Petroleum University,Chengdu 610500,China;Sinopec Group Zhongyuan Oil Prospecting Bureau,Puyang 457001,China)
出处
《广东化工》
CAS
2024年第10期28-32,共5页
Guangdong Chemical Industry
基金
中原油田博士后课题(2022BY04)。
关键词
细菌计数
绝迹稀释法
平板计数
ATP荧光快速检测法
细菌含量
bacterial count
extinction dilution method
plate count
ATP fluorescence rapid detection method
bacterial content
