摘要
目的:以美洲大蠊精制物CⅡ-3为研究对象,基于红外光谱结合化学计量学建立其品质无损的简易评价方法。方法:采用红外光谱法建立10批CⅡ-3样品的红外光谱,并进行基线矫正、纵坐标归一化和15点平滑等预处理方法优化光谱图;运用Origin 9.1软件对光谱数据进行表征和解析,建立10批CⅡ-3样品的指纹图谱,并进行相似度分析和主成分分析。结果:10批CⅡ-3样品红外光谱的官能团区主要特征峰为3300、2960、2930、1660、1630、1550、1450、1390 cm-1,指纹区主要特征峰为1240、1080、621、546 cm-1。不同批次CⅡ-3样品红外光谱的峰形、峰位和峰强相似,相关系数均在0.960以上。主成分分析结果显示10批CⅡ-3样品聚为一类。结论:该方法操作简单、专属性强、不破坏样品,可实现CⅡ-3样品的无损质量评价。
Objective:To establish a simple and nondestructive evaluation method of CⅡ-3 extract from Periplaneta americana based on infrared spectrum and chemometrics.Methods:Infrared spectra of 10 batches of CⅡ-3 samples were established by infrared spectroscopy,and the spectra were optimized by baseline correction,ordinate normalization and 15-point smoothing.Origin 9.1 software was used to characterize and analyze the spectral data,and the fingerprints of 10 batches of CⅡ-3 samples were established,and similarity analysis and principal component analysis were performed.Results:The main characteristic peaks of the functional group region in infrared spectra of 10 batches of CⅡ-3 samples were 3300,2960,2930,1660,1630,1550,1450,1390 cm-1.The main characteristic peaks in fingerprint region were 1240,1080,621,546 cm-1.The peak shape,position and intensity of infrared spectra were similar among different batches,and the correlation coefficients were all above 0.960.The results of principal component analysis showed that 10 batches of samples were clustered into one category.Conclusion:The method has the advantages of simple operations,strong specificity,no damage to the sample,and can be used for the quality evaluation for CⅡ-3 samples.
作者
周姣姣
夏从龙
李文璨
王艳平
李伟
何正春
Zhou Jiaojiao;Xia Congong;Li Wencan;Wang Yanping;Li Wei;He Zhengchun(Department of Pharmacy,Xiangtan Municipal Central Hospital,Xiangtan,Hunan 411100,China;College of Pharmacy,Dali University,Dali,Yunnan 671000,China)
出处
《大理大学学报》
2024年第8期41-45,共5页
Journal of Dali University
基金
中国药学会科技开发中心科普项目(CMEI2024KPYJ001176)
湘潭市医学科研项目(2023-xtyx-54)
国家自然科学基金项目(81660605)。
关键词
美洲大蠊
CⅡ-3
红外光谱
相似度分析
主成分分析
无损质量评价
Periplaneta americana
CⅡ-3
infrared spectroscopy
similarity analysis
principal component analysis
nondestructive quality evaluation
