摘要
目的 优化狐臭柴叶果胶酸法提取工艺,并评价果胶的理化性质、结构和抗氧化性。方法 以狐臭柴叶干粉为原料,采用酸法提取狐臭柴叶果胶,筛选出提取率较高的酒石酸为提取剂进行单因素实验和响应面优化,并分析最终产品的理化性质、抗氧化活性及结构特征。结果 各因素对果胶提取率的影响依次为:温度>p H>料液比>时间,最佳提取条件为:料液比1:60 (g/m L)、温度96℃、时间63 min、p H 1.60,在此条件下果胶提取率为27.10%,所得果胶为高酯果胶,其半乳糖醛酸含量、酸不溶灰分及干燥减量均符合GB25533—2010《食品安全国家标准食品添加剂果胶》要求。狐臭柴叶果胶的抗氧化活性与果胶浓度呈正相关,1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和羟基自由基(·OH)的清除率分别为61.0%和53.2%。此外,扫描电镜显示果胶呈块状,结构疏松。结论 采用酒石酸提取狐臭柴叶果胶的提取率较高,且果胶具有较好的抗氧化活性。
Objective To optimize the acid extraction process of pectin from Premna puberula Pamp.leaves and evaluate the physicochemical properties,structure,and antioxidant properties of pectin.Methods Using dried powder of Premna puberula Pamp.leaves as raw material,acid method was used to extract pectin from Premna puberula Pamp.leaves.Tartaric acid with high extraction rate was selected as the extraction agent for single factor experiments and response surface optimization.The physical and chemical properties,antioxidant activity,and structural characteristics of the final product were analyzed.Results The influence of various factors on the extraction rate of pectin had a size relationship,and the order was as follows:Temperature>pH>solid-liquid ratio>time.The optimal extraction conditions were:Solid-liquid ratio 1∶60(g/mL),temperature 96℃,time 63 minutes,pH 1.60.Under these conditions,the extraction rate of pectin was 27.10%.The resulting pectin was a high-methoxyl pectins,and its content of galactonic acid,acid insoluble ash,and drying reduction all meet the requirements of GB 25533-2010 National food safety standard-Food additive-Pectin.The antioxidant activity of pectin from Premna puberula Pamp.leaves was positively correlated with pectin concentration,and the scavenging rates of l,l-diphenyl-2-picrylhydrazyl(DPPH)free radicals and OH were 61.0%and 53.2%,respectively.Additionally,through observing by scanning electron microscope,the extracted pectin was blocky and had a loose structure.Conclusion Using tartaric acid to extract pectin from Premna puberula Pamp.leaves has a high extraction rate,and pectin had good antioxidant activity.
作者
黄海东
杨宁线
吴洪丽
谢霆霆
吕巩固
刘淼
张明生
HUANG Hai-Dong;YANG Ning-Xian;WU Hong-Li;Xie Ting-Ting;LV Gong-Gu;LIU Miao;ZHANG Ming-Sheng(College of Life Sciences/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guizhou University,Guiyang 550025,China;College of Public Health,Guizhou MedicalUniversity,Guiyang550025,China)
出处
《食品安全质量检测学报》
CAS
2024年第13期309-317,共9页
Journal of Food Safety and Quality
基金
贵州省山地农业关键核心技术攻关项目(GZNYGJHX-2023011)
贵州省中药材现代产业技术体系建设项目(GZCYTX-02)。
关键词
狐臭柴
果胶
酸法提取
理化性质
抗氧化活性
Premna puberula Pamp.
pectin
acid extraction
physical and chemical properties
antioxidant activity
