摘要
[目的]乙酰化葡萄糖是一种新型绿色盐碱土改良剂,研究乙酰化葡萄糖对盐碱土土壤肥力和细菌群落的影响,探索乙酰化葡萄糖改良盐碱土的可能作用机制,可为采用乙酰化葡萄糖改良盐碱地提供理论依据。[方法]选取山西省定襄县盐碱土为供试土壤,进行大豆盆栽试验,设置了对照(CK)、3个水平乙酰化葡萄糖处理T1(0.31 g·kg^(-1))、T2(0.62 g·kg^(-1))、T3(0.92 g·kg^(-1))和3个水平葡萄糖处理T4(0.31 g·kg^(-1))、T5(0.62 g·kg^(-1))、T6(0.92 g·kg^(-1)),分析比较不同处理大豆农艺性状、土壤化学性质及酶活性变化,并采用高通量测序技术对大豆根际土壤细菌16S rRNA基因V3-V4区进行多样性测序和分析。[结果]施用乙酰化葡萄糖后,大豆株高、主茎节数、地上部干重、根干重、单株粒数和单株粒重均显著高于对照。土壤pH和电导率分别比对照显著降低了0.21~0.34和8.91%~16.74%,土壤阳离子交换量、有机质含量则显著升高了8.52%~16.43%、4.78%~74.82%;土壤脲酶、碱性磷酸酶、过氧化氢酶活性显著增强。大豆完熟期根际土壤放线菌门的相对丰度显著提高;在属水平上,节杆菌属、斯科曼氏菌属、考克氏菌属的相对丰度显著增加。相关性分析结果显示,节杆菌属、斯科曼氏菌属与土壤pH、电导率显著负相关,与土壤有机质、阳离子交换量显著正相关;考克氏菌属也与土壤pH显著负相关,与阳离子交换量显著正相关。[结论]施用乙酰化葡萄糖能有效降低供试盐碱土碱性和土壤含盐量,提高土壤肥力和营养物质保持能力,改善根际土壤细菌群落结构,促进土壤中有益菌的活动,并进一步促进大豆生长,对于盐碱土改良具有明显作用。
[Objective]The study aimed to examine the impacts of acetylated glucose on soil fertility and bacterial community structure in saline-alkaline soil,and to investigate its potential mechanisms for soil improvement.The findings aimed to establish a theoretical support for using acetylated glucose to ameliorate saline-alkaline land.[Methods]Saline-alkaline soil from Dingxiang County,Shanxi Province,was selected for a soybean pot experiments.The experiment included a control(CK)and three levels of acetylated glucose treatment:T1(0.31 g·kg^(-1)),T2(0.62 g·kg^(-1)),and T3(0.92 g·kg^(-1)),as well as three levels of glucose treatments:T4(0.31 g·kg^(-1)),T5(0.62 g·kg^(-1)),and T6(0.92 g·kg^(-1)).The study analyzed and compared soybean agronomic traits,soil chemical properties,and enzyme activities under different treatments.High-throughput sequencing technology was used to sequence and analyze the diversity of the 16S rRNA gene V3-V4 region of bacterial communities in the soybean rhizosphere soil.[Results]The application of acetylated glucose significantly increased soybean plant height,main stem node number,aboveground dry weight,root dry weight,the number of grains per plant,and grain weight per plant compared to the control.Soil pH and electrical conductivity were significantly reduced by 0.21 to 0.34 and 8.91%to 16.74%,respectively,compared to the control.Soil cation exchange capacity and organic matter content were significantly elevated by 8.52%to 16.43%,and 4.78%to 74.82%,respectively.Soil urease,alkaline phosphatase,and catalase activities were all significantly enhanced.In the rhizosphere soil of mature soybeans,the relative abundance of Actinobacteria phylum increased significantly.At the genus level,the relative abundance of Arthrobacter,Skermanella and Kocuria increased significantly.Correlation analysis indicated that Arthrobacter and Skermanella were significantly negatively correlated with soil pH and electrical conductivity,and significantly positively correlated with soil organic matter and cation exchange capacity.Similarly,Kocuria was also significantly negatively correlated with soil pH and significantly positively correlated with cation exchange capacity.[Conclusion]The application of acetylated glucose effectively mitigated the alkalinity and salinity of the tested saline-alkaline soil,enhanced soil fertility and nutrient retention,improved the bacterial community structure in the rhizosphere soil,and promoted the activity of beneficial bacteria,and further facilitated soybean growth.These findings underscore the significant potential of acetylated glucose on the improvement of saline-alkaline soil.
作者
贾俊仙
高浩竣
李睿琦
殷丛丛
岳爱琴
杜维俊
赵晋忠
Jia Junxian;Gao Haojun;Li Ruiqi;Yin Congcong;Yue Aiqin;Du Weijun;Zhao Jinzhong(Department of Bacsic,Shanxi Agricultural University,Jinzhong 030800,China;College of Agronomy,Shanxi Agricultural University,J inzhong 030800,China)
出处
《山西农业大学学报（自然科学版）》
CAS
北大核心
2024年第4期82-92,共11页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
山西省科技成果转化引导专项计划(202104021301038)
山西省专利转化专项计划项目(202202035)。
关键词
盐碱土
乙酰化葡萄糖
土壤肥力
土壤细菌群落
Saline-alkaline soil
Acetylated glucose
Soil fertility
Soil bacterial community
