摘要
该研究以脱脂奶粉及核桃油为主要原料,以高产甾醇热带假丝酵母(Candida tropicalis)1253、保加利亚乳杆菌(Lactobacillus bulgaricus)及嗜热链球菌(Streptococcus thermophilus)为发酵菌种制备甾醇冻干粉,并在此基础上研制富含甾醇调制乳。以甾醇产量为评价指标,通过单因素试验和响应面试验优化菌株1253发酵工艺,对富含甾醇调制乳品质和营养进行综合性评价,并分析其关键差异代谢物及代谢通路。结果表明,最佳发酵工艺为培养时间97 h,培养温度30℃,菌种接种量9.5%,核桃油添加量4%。在此优化条件下,甾醇产量为0.90 mg/g。富含甾醇调制乳感官评分为(86.44±2.06)分,甾醇含量为(22.7±0.6)mg/g。吡哆醛、胆甾醇、γ-氨基丁酸(GABA)、麦角甾苷和柠檬酸等关键差异代谢物通过富集维生素B6代谢、磷酸戊糖途径、乙氧基化物和二甲酸酯代谢、果糖和甘露糖代谢和柠檬酸循环等途径形成特征风味。
In this study,using defatted milk powder and walnut oil as main raw materials,the freeze-dried sterol powder was prepared with high-yield sterol Candida tropicalis 1253,Lactobacillus bulgaricus and Streptococcus thermophilus as fermentation strains.On this basis,the sterol-rich modified milk was prepared.Using sterol yield as evaluation index,the fermentation process of strain 1253 was optimized by single factor tests and response surface tests.The quality and nutrition of sterol-rich modified milk was evaluated comprehensively,and the key differential metabolites and metabolic pathways were analyzed.The results showed that the optimum fermentation technology was culture time 97 h,temperature 30℃,inoculum 9.5%,walnut oil addition 4%.Under the optimized conditions,the sterol yield was 0.90 mg/g.The sensory score of sterol-enriched modified milk was(86.44±2.06)and the sterol content was(22.7±0.6)mg/g.Key differential metabolites such as pyridoxal,cholesterol,gamma-aminobutyric acid(GABA),acteoside and citric acid formed the characteristic flavors through enrichment of vitamin B6 metabolism,pentose phosphate pathway,ethoxylate and dicarboxylate metabolism,fructose and mannose metabolism,and citric acid cycle.
作者
杨婷婷
曾秋月
余春雨
许鑫业
田洋
黄艾祥
YANG Tingting;ZENG Qiuyue;YU Chunyu;XU Xinye;TIAN Yang;HUANG Aixiang(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Engineering Research Center for the Development and Utilization of Food and Medicine Homologous Resources,Kunming 650201,China;Yunnan Province Medicine and Food Homologous Functional Food Engineering Research Center,Kunming 650201,China;Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Kunming 650201,China)
出处
《中国酿造》
CAS
北大核心
2024年第8期133-143,共11页
China Brewing
基金
云南省重大科技项目(202102AE090027-1)
云南省省市一体化专项(A3032023057)
云岭产业技术领军人才项目(云发改人事[2014]1782号)。
关键词
高产甾醇热带假丝酵母
发酵工艺
富含甾醇调制乳
品质分析
差异代谢物
high-yield sterol Candida tropicalis
fermentation process
sterol-rich modified milk
quality analysis
differential metabolites
