摘要
目的探究箭叶淫羊藿Epimedium sagittatum(Sieb.et Zucc.)Maxim.叶片不同发育时期黄酮类成分变化与元素积累的规律,揭示其黄酮类成分的形成机制。方法以箭叶淫羊藿叶片为研究对象,将其按照不同发育时期分为t1~t6共6个时期,高效液相色谱(HPLC)测定4种黄酮类成分含量,紫外分光光度法测定总黄酮醇苷含量;实时荧光定量法(RT-qPCR)测定17个与黄酮类成分合成关键酶基因的相对表达量;电感耦合等离子体质谱法(ICP-MS)测定25种元素含量;皮尔逊(Pearson)相关性分析黄酮类成分与元素的关系。结果淫羊藿苷含量呈现先上升后下降的趋势,朝霍定A、朝霍定B、朝霍定C、总黄酮醇苷的含量显著降低,但是5种黄酮类成分总量却显著升高,在t4和t5时期大量积累。大部分黄酮类成分合成关键酶基因表达量在t4和t5时期显著上调,在t2时期显著下调,不同基因的表达量随叶片发育而变化;叶片中元素Mg、Ca、Ti、Mn、Fe、Sr和Ba元素含量显著升高,元素K和Co含量显著降低;元素P、K、V、Cu的含量与箭叶淫羊藿中黄酮类成分含量呈显著性正相关。结论箭叶淫羊藿叶片中黄酮类成分形成过程受到叶片发育程度及相关基因表达的影响,黄酮类成分在叶片发育后期大量积累。
Objective To explore the changes of flavonoids and the accumulation of elements in the leaves of Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.]at different developmental stages and to reveal the formation mechanism of flavonoids.Method The leaves of Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.]were divided into six stages from t1 to t6 according to different developmental stages,the contents of four flavonoids were determined by HPLC.The contents of total flavonol glycosides were determined by UV spectrophotometry.The relative expression of 17 genes related to flavonoid biosynthesis key enzyme genes were determined by qRT-PCR method and the contents of 25 elements were determined by ICP-MS.Pearson correlation analysis was used to analyze the relationship between flavonoids and elements.Results With the development of leaves,the contents of epimedin A,B,C and total flavonol glycosides decreased significantly while the content of icariin increased at first and then decreased.However,the total amount of five flavonoids increased significantly and accumulated in t4 and t5 periods.The relative expressions were significantly up-regulated at t4 and t5,and decreased significantly at t2,the expression of different genes varied with leaf development.The contents of Mg,Ca,Ti,Mn,Fe,Sr and Ba increased significantly,while the contents of K and Co decreased significantly.There was a significant positive correlation between the contents of P,K,V,Cu and the contents of flavonoids in Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.].Conclusion The formation of flavonoids in Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.]leaves is affected by the degree of leaf development and the expression of related genes,and flavonoids is accumulated in the late stage of leaf development.
作者
周省委
陈璐
董宁
乔璐
钟华
杨林林
ZHOU Shengwei;CHEN Lu;DONG Ning;QIAO Lu;ZHONG Hua;YANG Linin(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China;Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials,Zhengzhou 450046,Henan,China;Pingyu County Agriculture Rural Bureau,Zhumadian 463400,Henan,China)
出处
《中华中医药学刊》
CAS
北大核心
2024年第8期173-179,I0028,I0029,I0030,I0031,I0032,共12页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(82104329)
河南省重点研发与推广专项(科技攻关)(222102310182)。
关键词
箭叶淫羊藿
黄酮类成分
基因相对表达量
元素
组间相关性分析
Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.]
flavonoids
relative gene expression
elements
correlation analysis between groups
