摘要
目的研究金银花化学成分及其抗氧化活性。方法金银花提取物采用D101大孔树脂、硅胶柱和半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用DPPH自由基清除法测定其抗氧化活性。结果从中分离得到15个化合物,分别鉴定为dearabinosyl pneumonanthoside(1)、5α-carboxystrictosidine(2)、芹菜素-7-O-葡萄糖苷(3)、咖啡酸乙酯(4)、异鼠李素-3-O-β-D-葡萄糖苷(5)、木犀草素-7-O-葡萄糖苷(6)、异槲皮苷(7)、木犀草素-7-O-芸香糖苷(8)、木犀草素-7-O-新橙皮苷(9)、次大风子素(10)、绿原酸甲酯(11)、(Z)-aldosecolohanin(12)、kaempferol 3-O-β-D-glucoside(13)、绿原酸丁酯(14)、(-)-syringaresinol(15)。化合物3、5~10、13~14清除DPPH自由基的IC 50值为6.70~36.25μmol/L。结论化合物1~2、8~9、11和15为首次从忍冬属植物中分离。化合物3、5~10、13~14具有较好的抗氧化活性。
AIM To study the chemical constituents from Lonicera japonica Thunb.and their antioxidant activities.METHODS The extract from L.japonica was isolated and purified by D101 macroporous resin,silica gel column and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antioxidant activity was determined by DPPH free radical scavenging method.RESULTS Fifteen compounds were isolated and identified as dearabinosyl pneumonanthoside(1),5α-carboxystrictosidine(2),apigenin 7-O-glucoside(3),ethyl caffeate(4),isorhamnetin-3-O-β-D-glucopyranoside(5),luteolin 7-O-glucoside(6),quercetin 3-O-β-glucoside(7),luteolin 7-O-rutinoside(8),luteolin-7-O-neohesperidoside(9),hydnocarpin(10),methyl chlorogenate(11),(Z)-aldosecolohanin(12),kaempferol 3-O-β-D-glucoside(13),chlorogenic acid butyl ester(14),(-)-syringaresinol(15).The IC 50 values of DPPH free radical scavenging of compounds 3,5-10,13-14 were 6.70-36.25μmol/L.CONCLUSION Compounds 1-2,8,9-11 and 15 are isolated from genus Lonicera for the first time.Compounds 3,5-10,13-14 show good antioxidant activities.
作者
陆远
张研
王莹
董佳璇
杜宝玉
张永清
杨然
LU Yuan;ZHANG Yan;WANG Ying;DONG Jia-xuan;DU Bao-yu;ZHANG Yong-qing;YANG Ran(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第8期2638-2644,共7页
Chinese Traditional Patent Medicine
基金
中央引导地方科技发展资金项目(XZ202301YD0023C)
中央本级重大增减支项目(2060302)
山东省重点研发计划(2021CXGC010510,2022CXGC010511)
山东中医药大学药学院青年教师科研强基计划(2021-0012,2021-0015)。
关键词
金银花
化学成分
分离鉴定
抗氧化活性
Lonicera japonica Thunb.
chemical constituents
isolation and identification
antioxidant activities
