摘要
目的探讨寻常型银屑病病人血清微RNA(miR)-138、miR-32水平变化与炎症反应、疾病活动性、预后转归的关系。方法选取2021年7月至2022年6月在沧州市人民医院进行治疗的寻常型银屑病病人80例作为观察组,根据疾病活动性分期将观察组80例寻常型银屑病病人分为活动期46例、稳定期34例,另选取同期整形外科体检健康者40例作为对照组。检测血清miR-138、miR-32表达水平、炎症因子[白细胞介素(IL)-4、IL-8、肿瘤坏死因子-α(TNF-α)]水平;采用相关性分析血清miR-138、miR-32水平与炎症因子的关系、血清miR-138、miR-32水平与病人疾病活动性和银屑病皮损面积和严重程度指数(PASI)评分的关系;采用受试者操作特征曲线(ROC曲线)分析血清miR-138、miR-32诊断病人疾病活动性的价值。结果与对照组相比,观察组病人血清miR-138水平降低(0.67±0.19比0.98±0.21),miR-32(1.26±0.37比0.96±0.18)、IL-4、IL-8和TNF-α水平升高,差异有统计学意义(P<0.05);与稳定期病人相比,活动期病人血清miR-138水平降低(0.61±0.10比0.75±0.14),miR-32水平(1.34±0.35比1.16±0.29)和PASI评分升高,差异有统计学意义(P<0.05);治疗前、治疗后2周和治疗后4周病人血清miR-138水平依次升高(0.67±0.19,0.78±0.21,0.89±0.27),miR-32水平(1.26±0.37,1.14±0.33,1.02±0.28)和PASI评分依次降低(P<0.05);血清miR-138水平与IL-4、IL-8、TNF-α、疾病活动性和PASI评分呈负相关(P<0.05),血清miR-32水平与IL-4、IL-8、TNF-α、疾病活动性和PASI评分呈正相关(P<0.05);miR-138和miR-32二者联合检测诊断病人疾病活动性的曲线下面积为0.86,灵敏度为84.78%,特异度为85.29%。结论寻常型银屑病病人血清miR-138水平下调,miR-32水平上调,二者水平变化与炎症反应、疾病活动性和预后转归有关,且联合检测对诊断疾病活动性具有一定的价值。
Objective To investigate the serum miR-138 and miR-32 levels in patients with psoriasis vulgaris and their relationship with inflammatory reaction,disease activity and prognostic outcome.Methods Eighty cases of patients with psoriasis vulgaris who were treated in Cangzhou People's Hospital from July 2021 to June 2022,were selected as the observation group.According to the disease activity staging,the patients were assigned into active stage ones(n=46)and stable stage ones(n=34),and another 40 healthy people having a check-up in Department of Plastic Surgery during the same period were selected as the control group.Serum miR-138,miR-32 expression levels,and levels of inflammatory factors[interleukin(IL)-4,IL-8 and tumor necrosis factor-α(TNF-α)]were examined;correlation was applied to analyze the relationship between serum miR-138,miR-32 levels and inflammatory factors,and the relationship between serum miR-138,miR-32 levels and disease activity and psoriasis area and severity index(PASI)score.The value of serum miR-138 and miR-32 in diagnosing the disease activity of patients was analyzed by using receiver operating characteristic(ROC).Results Compared with the control group,serum miR-138 level(0.67±0.19 vs.0.98±0.21)was decreased and miR-32(1.26±0.37 vs.0.96±0.18),IL-4,IL-8 and TNF-αlevels were increased in the observation group,and the differences were statistically significant(P<0.05).Compared with patients in the active stage,serum miR-138 level(0.61±0.10 vs.0.75±0.14)were lower and miR-32 level(1.34±0.35 vs.1.16±0.29)and PASI score were higher in the active stage patients,with statistically significant differences(P<0.05).The levels of serum miR-138(0.67±0.19,0.78±0.21,0.89±0.27)of patients before treatment,2 weeks after treatment and 4 weeks after treatment increased in turn,and the levels of miR-32(1.26±0.37,1.14±0.33,1.02±0.28)and PASI score decreased in turn(P<0.05).The level of serum miR-138 was negatively correlated with IL-4,IL-8,TNF-α,disease activity and PASI score(P<0.05),while the level of serum miR-32 was positively correlated with IL-4,IL-8,TNF-α,disease activity and PASI score(P<0.05).The area under the curve of miR-138 combined with miR-32 to detect the disease activity of patients was 0.86,the sensitivity was 84.78%,and the specificity was 85.29%.Conclusion In patients with psoriasis vulgaris,the level of serum miR-138 is down regulated and the level of miR-32 is up regulated,the changes in both levels are related to inflammatory reaction,disease activity and prognostic outcome,and the combined detection has certain value in diagnosing disease activity.
作者
谢艳飞
滕伟
刘朋
李二净
张群
潘祖爱
XIE Yanfei;TENG Wei;LIU Peng;LI Erjing;ZHANG Qun;PAN Zuai(Department of Dermatology,Cangzhou People's Hospital,Cangzhou,Hebei 061000,China;Department of Plastic Surgery,Cangzhou People's Hospital,Cangzhou,Hebei 061000,China)
出处
《安徽医药》
CAS
2024年第9期1823-1827,共5页
Anhui Medical and Pharmaceutical Journal
基金
2022年度河北省医学科学研究课题计划项目(20220306)。
