摘要
目的分析Toll样受体2(TLR2)及Toll样受体4(TLR4)mRNA表达水平与前列腺癌(PC)根治术后尿路感染发生及转归的关系。方法选取2020年3月至2023年3月衡水市第二人民医院收治的80例PC根治术后尿路感染患者作为感染组,另选取同期80例PC根治术后未发生尿路感染患者作为非感染组。比较两组TLR2、TLR4 mRNA相对表达量及血清炎症因子水平,分析TLR2、TLR4 mRNA表达量与炎症因子的相关性;根据感染组治疗后转归情况分为预后良好组和预后不良组,分析TLR2、TLR4 mRNA对不良预后的预测效能。结果与非感染组比较,感染组外周血单个核细胞TLR2、TLR4 mRNA表达量及血清白介素(IL)-6、IL-8、肿瘤坏死因子-α(TNF-α)水平更高(P<0.05);Pearson分析显示,TLR2、TLR4 mRNA表达量与血清IL-6、IL-8、TNF-α水平均呈正相关(P<0.05);感染组治疗后61例预后良好(预后良好组),其外周血单个核细胞TLR2、TLR4 mRNA表达量较预后不良组更高(P<0.05);TLR2、TLR4 mRNA单独及两者联合预测尿路感染不良预后的灵敏度/特异度分别为89.50%/63.90%、73.70%/73.80%、94.70%/62.30%,曲线下面积(AUC)分别为0.808、0.790、0.890。结论PC根治术后尿路感染的发生及转归与外周血TLR2、TLR4 mRNA表达有关,其作用机制可能为上调IL-6、IL-8、TNF-α等炎症因子表达。
Objective To analyze the relationship between mRNA expression levels of Toll-like receptor 2(TLR2)and Toll-like receptor 4(TLR4)and occurrence and outcomes of urinary tract infection after radical surgery of prostate cancer(PC).Methods A total of 80 patients with urinary tract infection after PC radical surgery admitted to the Second People′s Hospital of Hengshui from March 2020 to March 2023 were selected as study objects and set as infected group,and another 80 patients who did not develop urinary tract infection after PC radical surgery during the same period were selected and set as non-infected group.The mRNA expression levels of TLR2 and TLR4 and the levels of serum inflammatory factors were compared between the two groups.The correlation between mRNA expression levels of TLR2 and TLR4 and inflammatory factors was analyzed.According to the outcomes of patients in infected group after treatment,they were divided into good prognosis group and poor prognosis group,and the predictive efficiency of TLR2 mRNA and TLR4 mRNA on poor prognosis was analyzed.Results The mRNA expression levels of TLR2 and TLR4 in peripheral blood mononuclear cells and levels of serum interleukin(IL)-6,IL-8 and tumor necrosis factor-α(TNF-α)were higher in infected group compared with those in non-infected group(P<0.05).Pearson analysis showed that mRNA expression levels of TLR2 and TLR4 were positively correlated with levels of serum IL-6,IL-8 and TNF-α(P<0.05).After treatment,there were 61 patients with good prognosis in infected group(good prognosis group).Compared with poor prognosis group,the mRNA expression levels of TLR2 and TLR4 in peripheral blood mononuclear cells were higher in good prognosis group(P<0.05).The sensitivities/specificities of TLR2 mRNA,TLR4 mRNA alone and combined detection in predicting poor prognosis of urinary tract infection were 89.50%/63.90%,73.70%/73.80%and 94.70%/62.30%,and the areas under the curves(AUC)were 0.808,0.790 and 0.890 respectively.Conclusions The occurrence and outcomes of urinary tract infection after PC radical surgery are related to the mRNA expressions of TLR2 and TLR4 in peripheral blood,and their role mechanism may be related to the up-regulations of expressions of IL-6,IL-8,TNF-αand other inflammatory factors.
作者
程刚
罗伯峰
田寒松
CHENG Gang;LUO Bofeng;TIAN Hansong(Department of Urinary Surgery,the Second People′s Hospital of Hengshui,Hengshui 053000,Hebei,China)
出处
《中国性科学》
2024年第8期35-38,共4页
Chinese Journal of Human Sexuality
关键词
TOLL样受体2
TOLL样受体4
炎症因子
前列腺癌根治术
尿路感染
预后
Toll-like receptor 2
Toll-like receptor 4
Inflammatory factors
Radical surgery of prostate cancer
Urinary tract infection
Prognosis
