摘要
柠檬酸作为柠檬酸生产废水(CAPW)的主要成分之一,具有作为强化污水生物脱氮补充碳源的潜质.本研究以传统碳源乙酸钠作为对照,考察了柠檬酸钠强化低C/N校园生活污水生物脱氮的效果,研究了碳源和氮素的去除效果及降解规律、活性污泥性能和微生物群落结构.结果表明,当分别投加柠檬酸钠和乙酸钠作为补充碳源,调节校园生活污水C/N为4时,总氮去除率从未补充碳源时的47.1%和47.0%分别提高到98.5%和97.6%,且反硝化动力学指标相近.以柠檬酸钠为补充碳源的反应器中,反硝化过程中没有NO2--N积累,而乙酸钠反应器中出现NO2--N短暂积累.柠檬酸钠反应器中的活性污泥展现了更佳的沉降性能,污泥体积指数(SVI)值比乙酸钠系统中低42.83 mL·g^(-1),这与污泥胞外聚合物(EPS)总量、松散结合型EPS(LB-EPS)含量、紧密结合型EPS(TB-EPS)中蛋白含量低有关.柠檬酸钠反应器中亚硝酸盐还原菌Saprospiraceae相对丰度较高(17.70%),而乙酸钠反应器中硝酸盐还原菌Rhodocyclaceae相对丰度较高(24.83%).柠檬酸作为补充碳源用于强化污水生物脱氮具有优势.
As one of the main components of citric acid production wastewater(CAPW),the citric acid had a potential to be used as additional carbon source to enhance biological nitrogen removal for wastewater.In this study,the performance of citrate on enhancing nitrogen removal for low C/N ratio campus domestic wastewater was investigated,using the traditional carbon source acetate for comparison.The removal efficiencies and degradation rules of carbon and nitrogen,characteristics of activated sludge and microbial community structures were studied.Results showed that using sodium citrate and sodium acetate as additional carbon sources to adjust the C/N ratio to 4,the total nitrogen(TN)removal efficiencies increased from 47.1%and 47.0%to 98.5%and 97.6%,respectively,and the denitrification kinetics indicators were similar.In the reactor with citrate as the additional carbon source,there was no NO2--N accumulation during denitrification,while a transient accumulation of NO2--N occurred in the reactor fed with acetate.The activated sludge in the citrate reactor had better settling property,and the sludge volume index(SVI)value was 42.83 mL·g^(-1) lower than that in the acetate reactor,which was related to the lower total amount of extracellular polymer(EPS),lower content of loosely bound EPS(LB-EPS)and lower protein content in the tightly bound EPS(TB-EPS).Saprospiraceae had higher relative abundance(17.70%)in the reactor fed with citrate,while Rhodocyclaceae(24.83%)in the reactor fed with acetate.Using citrate as the additional carbon source had advantages in enhancing biological nitrogen removal for wastewater.
作者
孙世佳
李茹莹
孙邓威
SUN Shijia;LI Ruying;SUN Dengwei(College of Environmental Science and Engineering,Tianjin University,Tianjin 300350;Tianjin Municipal Engineering Design&Research Institute Co.Ltd.,Tianjin 300392)
出处
《环境科学学报》
CAS
CSCD
北大核心
2024年第8期170-180,共11页
Acta Scientiae Circumstantiae
基金
国家水体污染控制与治理科技重大专项(No.2017ZX07106004)。
关键词
生物脱氮
生活污水
碳源
柠檬酸
微生物群落结构
biological nitrogen removal
domestic wastewater
carbon source
citric acid
microbial community structure
