摘要
目的 对土炒白术的炮制工艺进行优化。方法 采用紫外可见分光光度法和超高效液相色谱-三重四极杆串联质谱(UPLC-QqQ-MS/MS)技术对土炒白术中多糖含量以及白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ等活性成分的含量进行测定,采用色差仪对土炒白术粉末色度值进行分析;将上述化学成分的含量以及饮片的色度作为评价指标,采用单因素试验法对土炒白术过程中的辅料用量、炒制温度、炒制时间进行考察;通过层次分析-赋值(AHP-CRITIC)混合加权法结合BoxBehnken响应面法进行土炒白术炮制工艺的优化研究。结果 优化土炒白术的最佳炮制工艺为每50 g药材用灶心土28.4 g,在204℃下炒制5.30 min。结论 本实验确定的土炒白术工艺切实可行,可为该炮制品的工业化生产提供一定的指导意义。
OBJECTIVE To optimize the processing technology of soil stir-fried Atractylodis Macrocephalae Rhizoma(AMR).METHODS UV-visible spectrophotometry and ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QqQ-MS/MS)was used to determine the content of polysaccharides and active ingredients such as atractylenolideⅠ,atractylenolideⅡ,atractylenolideⅢ,etc.The colorimeter was used to determine the color value of soil-fried AMR powder.The contents of the above chemical components and the color value of soil-fried AMR were used as evaluation indexes.The single factor trial method was adopted to investigate the amount of excipients,stir-frying temperature and time of AMR.The analytic hierarchy process and criteria importance through inter criteria correlation(AHP-CRITIC)combined with the method of Box-Behnken response surface was used to design and optimize the processing technology of soil stir-fried AMR.RESULTS The best processing technology of soil fried AMR were optimized to 28.4 g of soil added to 50 g of medicinal materials which was fried 5.30 min in 204℃.CONCLUSION The process of soil stir-fried AMR determined in this experiment is feasible which can provide certain guiding significance for the industrial production of the processed products.
作者
袁楚
刘想
高如汐
王凡一
张可
吴昊
刘思奇
赵启苗
单国顺
YUAN Chu;LIU Xiang;GAO Ruxi;WANG Fanyi;ZHANG Ke;WU Hao;LIU Siqi;ZHAO Qimiao;SHAN Guoshun(Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Liaoning Vocational College of Medicine,Shenyang 110101,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2024年第14期1956-1964,共9页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金青年基金项目(81803726)
辽宁省教育厅面上项目(LJKMZ20222205,LJKMZ20221308)
辽宁中医药大学校级课题重点项目(2021 LZY054)
辽宁医药职业学院校级课题(BSKY202405)。
关键词
白术
土炒
混合加权法
Box-Behnken响应面法
工艺优化
Atractylodis Macrocephalae Rhizoma
soil-fried
mixed weighting method
Box-Behnken response surface method
process optimization
