摘要
目的 分析扁桃体切除对大鼠TH1/TH2免疫平衡及免疫蛋白表达的影响及可能的作用机制。方法 样本选取21只成年雄性大鼠,使用随机数字表法将所有大鼠分为对照组、囊内切除组及囊外切除组,对照组大鼠正常喂养,囊内组大鼠基于扁桃体囊内切除术,囊外组给予扁桃体囊外切除术。比较各组大鼠TH1/TH2细胞因子及TH1/TH2、CD3^(+)、CD4^(+)、CD8^(+)细胞水平、免疫蛋白水平表达,检测各组大鼠MCP-1、CCR2蛋白表达。结果 囊内组、囊外组TH1、TH2、TH1/TH2水平低于对照组且囊内组高于囊外组(P<0.05);囊内组、囊外组CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)水平低于对照组且囊内组高于囊外组(P<0.05);囊内组、囊外组IgA、IgG、IgM水平低于对照组且囊内组高于囊外组(P<0.05);与对照组相比,囊内组B细胞亚群变化低于囊外组(P<0.05);囊内切除对大鼠B细胞亚群影响更小且B1在每个B细胞亚群上表达,而囊外组B2、B3在B细胞亚群上的表达减少。B4在ASC上呈现唯一表达,囊外组的水平低于囊内组(P<0.05),比较平均荧光强度,囊内组ASC的B1、B4水平显著高于囊外组(P<0.05);囊内组、囊外组MCP-1、CCR2蛋白相对表达水平高于对照组且囊内组低于囊外组,差异具有统计学意义(P<0.05)。结论 扁桃体囊内、囊外切除大鼠均表现出免疫失衡及免疫细胞、免疫蛋白降低,但使用囊内切除有助于提高大鼠TH1/TH2细胞因子及TH1/TH2免疫平衡、免疫蛋白表达、CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)水平,其作用机制可能与MCP-1/CCR2蛋白通路调控有关。
Objective To analyze the effects of tonsillectomy on TH1/TH2 immune balance and immune protein expression in rats and its possible mechanism.Methods A total of 21 adult male rats were selected and di-vided into control group,intracapsular group and extracapsular group using random number table method.The rats in the control group were fed normally,the rats in the intracapsular group were given intracapsular resection,and the rats in the extracapsular group were given extracapsular resection.The levels of TH1/TH2 cytokines,TH1/TH2,CD3^(+),CD4^(+),CD8^(+)cells and immune protein in each group were compared,and the expression of MCP-1 and CCR2 proteins in each group were detected.Results The levels of TH1,TH2 and TH1/TH2 in intracapsular group and ex-tracapsular group were lower than those in control group,and those in intracapsular group were higher than those in extracapsular group.The levels of CD3^(+),CD4^(+),CD8^(+)and CD4^(+)/CD8^(+)in intracapsular group and extracapsular group were lower than those in control group,and those in intracapsular group were higher than those in extracapsular group.The levels of IgA,IgG and IgM in intracapsular and extracapsular groups were lower than those in control group,and the levels in intracapsular and extracapsular groups were higher than those in control group.Compared with the control group,the change of B cell subsets in the intracapsular group was lower than that in the extracapsu-lar group.Intracapsular resection had less effect on B-cell subsets and B1 was expressed on each B-cell subset,while the expression of B2 and B3 on B-cell subsets was decreased in extracapsular group.The expression of B4 was unique on ASC,and the level of B4 in the extracapsular group was lower than that in the intracapsular group.Com-pared with the average fluorescence intensity,the level of B1 and B4 in the intracapsular group was significantly higher than that in the extracapsular group.The relative expression levels of MCP-1 and CCR2 protein in intracapsu-lar group and extracapsular group were higher than those in control group,and those in intracapsular group were low-er than those in extracapsular group,with statistical significance(P<0.05).Conclusion Both intracapsular and ex-tracapsular tonsillectomy rats showed immune imbalance and decreased immune cells and immune proteins,but in-tracapsular tonsillectomy could improve TH1/TH2 cytokines,TH1/TH2immune balance,immune protein expres-sion,CD3^(+),CD4^(+),CD8^(+)and CD4^(+)/CD8^(+)levels in rats.The mechanism may be related to the regulation of MCP-1/CCR2 protein pathway.
作者
瞿帅
周汝环
张雁冰
李坤军
曹峰
QU Shuai;ZHOU Ru-huan;ZHANG Yan-bing;LI Kun-jun;CAO Feng(Guangde Road Campus of the Second People's Hospital of Hefei City,Hefei,Anhui Province 230011,China)
出处
《解剖学研究》
CAS
2024年第4期310-314,321,共6页
Anatomy Research
基金
安徽省高等学校科学研究(自然科学类)重点项目(2022AH050787)
合肥市卫生健康应用医学研究重点项目(Hwk2022zd009)。
