摘要
【目的】对薄壳山核桃Carya illinoensis害虫暗影饰皮夜蛾Garella ruficirra线粒体基因组进行测序和分析,并在基因组水平上探讨其在夜蛾科Noctuidae中的分类地位,为探索夜蛾科昆虫的系统发育关系以及演化进程提供参考。【方法】利用二代测序技术从头组装获取暗影饰皮夜蛾的线粒体基因组,并对线粒体基因组结构特点和碱基组成进行分析;同时,采用最大似然法和贝叶斯法联合构建了夜蛾科5个属、12个种的线粒体基因组系统发育树,分析暗影饰皮夜蛾在夜蛾科中的系统发育地位。【结果】暗影饰皮夜蛾线粒体基因组全长共为15294 bp,其中包括13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因以及鳞翅目Lepidoptera昆虫典型的腺嘌呤(A)+胸腺嘧啶(T),即A+T富含区,该区域的A+T含量为80.53%,具有明显的AT偏向性。暗影饰皮夜蛾的基因排列顺序为trnM-trnI-trnQ,与包括夜蛾科昆虫在内的大多数鳞翅目昆虫基因排列次序相符。13个蛋白质编码基因的起始密码子全部为ATN。22个tRNA基因中除trnS1的DHU臂缺失,其余均为典型的三叶草结构。对线粒体基因组研究发现:夜蛾科5个属之间,Garella与皮夜蛾属Nycteola亲缘关系最近,与饰夜蛾属Pseudoips亲缘关系最远。【结论】暗影饰皮夜蛾的线粒体基因组中出现了基因重排的现象,系统发育关系支持暗影饰皮夜蛾和Garella musculana聚为1个分支。
[Objective] This study, with a comprehensive sequencing and analysis of the mitochondrial genomeof Garella ruficirra as well as an investigation into the phylogenetic position of Noctuidae throughmitochondrial genome sequencing, is aimed to offer a valuable reference for delving into the phylogeneticrelationships and evolutionary processes within the Noctuidae as well as exploring the phylogenetic relationshipand evolutionary process of Noctuidae. [Method] First, the Illumina sequencing technique was employed tosequence the complete mitochondrial genome of G. ruficirra, allowing for the analysis of its overallcharacteristics and base composition. Subsequently, a phylogenetic tree encompassing mitochondrial genomesfrom 5 genera of Noctuidae, comprising 12 species, was constructed using both maximum likelihood andBayesian methods so as to facilitate the examination of the phylogenetic relationships within the Noctuidae.[Result] The circular mitochondrial genome of G. ruficirra spanned 15 294 base pairs and encompasses 13 protein synthesis-related genes, 22 transfer RNA genes, and two ribosomal RNA genes and its genearrangement conformed to the standard configuration observed in Lepidoptera mitogenomes, featuring a regionrich in adenine and thymine, an A+T content of 80.53% and a significant AT bias. The gene order of trnM-trnItrnQin G. ruficirra aligned with other Noctuidae species and the majority of Lepidoptera with ATN sequencesserving as initiation codons for all 13 protein-coding genes. While trnS1 lacked a DHU arm, the remainingtRNA genes exhibited a typical cloverleaf-shaped structure. According to the study of mitochondrial genome,Garella was most closely related to Nycteola and least closely related to Pseudoips among the 5 genera.[Conclusion] Gene rearrangement was evident in the mitochondrial genome of G. ruficirra. Phylogeneticanalyses, indicating that the clustering of G. ruficirra and G. musculana samples was within a single clade. Thisstudy has furnished valuable insights into the evolutionary patterns of Garella and offered a foundationalframework for investigating the phylogeny and evolution of Noctuidae within the Lepidoptera order.
作者
李妍
舒金平
华克达
张亚波
应玥
张威
LI Yan;SHU Jinping;HUA Keda;ZHANG Yabo;YING Yue;ZHANG Wei(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,Zhejiang,China;Hangzhou Fuyang District Agriculture and Rural Bureau,Hangzhou 311400,Zhejiang,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2024年第4期724-734,共11页
Journal of Zhejiang A&F University
基金
中国林业科学研究院基本科研业务费专项资金项目(CAFYBB2022SY011)。
关键词
暗影饰皮夜蛾
线粒体基因组
系统发育关系
基因重排
Garella ruficirra
mitochondrial genome
phylogenetic relationship
gene rearrangement
