摘要
目的探究miR-145-5p在原发性高血压(EH)患者血清中的表达及对人脐静脉内皮细胞(HUVEC)增殖和凋亡的影响。方法以2019年1月至2022年3月于山西医科大学汾阳学院就诊的160例EH患者及160例同期体检正常者为研究对象,RT-qPCR检测EH患者及同期体检正常者血清中的miR-145-5p表达水平。体外培养HUVEC,将之分为对照组、miR-145-5p mimic组、mimic-NC组、miR-145-5p inhibitor及inhibitor-NC组,CCK-8法检测各组HUVEC增殖情况;AnnexinV-FITC/PI双染法检测各组HUVEC凋亡情况;Western blot检测各组HUVEC增殖、凋亡相关蛋白[增殖细胞核抗原(PCNA)、细胞周期蛋白D1(Cyclin D1)、B细胞淋巴瘤/白血病基因-2相关X蛋白(Bax)、半胱氨酸天冬氨酸特异性蛋白酶-3(Caspase-3)]表达情况。结果miR-145-5p在EH患者血清中的表达水平明显低于体检正常者(P<0.05);与对照组、mimic-NC组比较,miR-145-5p mimic组HUVEC细胞增殖率及PCNA、Cyclin D1蛋白表达均增加,早期凋亡率、晚期凋亡率及Bax、Caspase-3蛋白表达均减少(P<0.05);与对照组、inhibitor-NC组比较,miR-145-5p inhibitor组HUVEC细胞增殖率及PCNA、Cyclin D1蛋白表达均减少(P<0.05),早期凋亡率、晚期凋亡率及Bax、Caspase-3蛋白表达均增加(P<0.05)。结论miR-145-5p在EH患者血清中呈低表达,过表达miR-145-5p可促进HUVEC增殖并抑制其凋亡。
Objective To investigate the expression of miR-145-5p in the serum of patients with essential hypertension(EH)and its impacts on the proliferation and apoptosis of human umbilical vein endothelial cells(HUVEC).Methods From January 2019 to March 2022,160 patients with EH and 160 patients with normal physical examination results who were treated in Fenyang College of Shanxi Medical University were selected as the research objects.RT-qPCR was applied to detect the expression level of miR-145-5p in serum of EH patients and healthy subjects.HUVECs were cultured in vitro and divided into control group,miR-145-5p mimic group,mimic-NC group,miR-145-5p inhibitor group and inhibitor-NC group.The proliferation of HUVEC in each group was detected by CCK-8 method;AnnexinV-FITC/PI double staining was applied to detect the apoptosis of HUVEC in each group;Western Blot was applied to detect the expressions of HUVEC proliferation and apoptosis-related proteins[proliferating cell nuclear antigen(PCNA),Cyclin D1,B-cell lymphoma/leukemia gene-2-associated X protein(Bax),Caspase-3]in each group.Results The expression level of miR-145-5p in serum of EH patients was significantly lower than that of patients with normal physical examination results(P<0.05).Compared with the con-trol group and the mimic-NC group,the proliferation rate of HUVEC cells and the protein expressions of PCNA and Cyclin D1 in the miR-145-5p mimic group were increased,while the early apoptosis rate,late apoptosis rate and the protein expressions of Bax and Caspase-3 were decreased(P<0.05).Compared with the control group and the inhibitor-NC group,the proliferation rate of HUVEC cells and the protein expressions of PCNA and Cyclin D1 in the miR-145-5p inhibitor group were decreased(P<0.05),while the early apoptotic rate,late apoptotic rate,the protein expressions of Bax and Caspase-3 were increased(P<0.05).Conclusion miR-145-5p was lowly expressed in the serum of EH patients,and overexpression of miR-145-5p could promote the proliferation and inhibit the apopto-sis of HUVEC.
作者
田朝霞
李卫萍
赵娜
李红梅
王全义
TIAN Zhaoxia;LI Weiping;ZHAO Na;LI Hongmei;WANG Quanyi(Department of Nursing Basic Education,Fenyang College of Shanxi Medical University,Lüliang 032200,Shanxi,China;Department of Pathology,Fenyang College of Shanxi Medical University,Lüliang 032200,Shanxi,China;Laboratory,Fenyang College of Shanxi Medical University,Lüliang 032200,Shanxi,China;Department of Nursing,Fenyang College of Shanxi Medical University,Lüliang 032200,Shanxi,China;Department of Cardiology,Shanxi Fenyang Hospital,Lüliang 032200,Shanxi,China)
出处
《医学研究与战创伤救治》
CAS
北大核心
2024年第5期504-508,共5页
Journal of Medical Research & Combat Trauma Care
基金
吕梁市重点研发项目(2020SHFZ49)。
关键词
miR-145-5p
原发性高血压
人脐静脉内皮细胞
增殖
凋亡
miR-145-5p
essential hypertension
human umbilical vein endothelial cells
proliferation
apoptosisdisorders
