摘要
为了阐明番茄红素(lycopene,LYC)对雏鹅运输应激的影响,揭示LYC保护作用的关键位点,明确LYC保护雏鹅运输应激的具体作用和分子机制,试验采用模拟运输的模型,将45只雏鹅随机分为对照组、运输应激模型组和LYC处理组,其中对照组不做任何处理,运输应激模型组进行持续6 h的摇床振荡,LYC处理组雏鹅按体重50 mg/kg灌胃LYC 1 h后再进行持续6 h的摇床振荡,对雏鹅进行安乐死处理,快速打开胸腔和腹腔,以脏采血,冰上剥离肝脏组织。通过H.E.染色、生化功能检测、ELISA、实时荧光定量RCR检测等技术,观察肝脏的病理组织学改变,测定生化指标和氧化应激指标及肝脏相关基因表达量。结果表明:与对照组比较,运输应激模型组天门冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶、乳酸脱氢酶活性及白蛋白、总胆红素含量均极显著升高(P<0.01);LYC处理组各指标均下降,与运输应激模型组比较差异极显著(P<0.01)。运输应激模型组雏鹅肝脏可见明显的细胞坏死、空泡变性、充血等病理损伤,LYC处理组肝脏损伤明显缓解。与对照组比较,运输应激模型组丙二醛含量显著升高(P<0.05),谷胱甘肽还原酶活性和ATP含量极显著降低(P<0.01),超氧化物歧化酶活性显著降低(P<0.05);与运输应激模型组比较,LYC处理组丙二醛含量显著降低(P<0.05),超氧化物歧化酶活性显著升高(P<0.05),谷胱甘肽和ATP含量极显著升高(P<0.01)。与运输应激模型组比较,LYC处理组氧化应激相关因子肝脏核因子E2(Nrf2)、血红素加氧酶-1(HO-1)及醌氧化还原酶1(NQO1)基因相对表达量极显著升高(P<0.01),Kelch样环氧氯丙烷相关蛋白1(Keap1)基因相对表达量极显著降低(P<0.01);炎性因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)基因相对表达量显著或极显著降低(P<0.05或P<0.01);肝脏凋亡基因B淋巴细胞瘤/白血病-2相关X蛋白(Bax)基因相对表达量极显著降低(P<0.01),B淋巴细胞瘤-2(Bcl-2)基因相对表达量极显著升高(P<0.01),Bax/Bcl-2极显著降低(P<0.01)。说明LYC可通过影响Nrf2/HO-1/NQO1信号通路提高雏鹅抗氧化能力并且可降低炎性因子的释放,同时影响Bcl-2、Bax等凋亡基因的表达,缓解运输应激对肝脏组织功能及实质的损伤。
The present study was conducted to elucidate the effects of lycopene(LYC) on transport stress(TS) in goslings and to reveal the key loci of the protective effects of LYC, as well as to clarify the specific roles and molecular mechanisms of LYC in protecting goslings against transport stress. A simulated transport model was used in the experiment, and 45 goslings were randomly divided into control group, transport stress model group and LYC treatment group. The control group did not receive any treatment, the transport stress model group was subjected to shaker oscillation for 6 h, and the goslings in the LYC treatment group were subjected to shaker oscillation for 6 h after gavage of LYC at the weight of 50 mg/kg for 1 h. The pathological changes of liver tissue were observed by H.E. staining method and liver biochemical function-test, ELISA, real-time fluorescence quantitative RCR detection, and liver function and oxidative stress indexes and liver-related gene expression were determined. The results showed that compared with the control group, the activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase and the contents of albumin and total bilirubin in the transport stress model group were significantly increased(P<0.01). All the indexes of LYC treatment group were decreased, and the difference was extremely significant compared with transport stress model group(P<0.01). In the transport stress model group, the liver of goslings showed obvious pathological damage such as cell necrosis, vacuolar degeneration and congestion, and the liver damage was significantly relieved after LYC treatment. Compared with the control group, malondialdehyde content in transport stress model group was significantly increased(P<0.05), glutathione and ATP contents were significantly decreased(P<0.01), and superoxide dismutase activity was significantly decreased(P<0.05). After LYC treatment, compared with transport stress model group, malondialdehyde content in LYC treatment group was significantly decreased(P<0.05);superoxide dismutase activity was significantly increased(P<0.05);glutathione and ATP contents were significantly increased(P<0.01). Meanwhile, the relative mRNA expression of oxidative stress-related factors in liver nuclear factor E2-related factor(Nrf2), heme oxygenase-1(HO-1) and quinone oxidoreductase 1(NQO1) was significantly increased after LYC treatment(P<0.05). The relative mRNA expression of kelch-like epichlorohydrin-associated protein 1(Keap1) was significantly decreased(P<0.01). The relative mRNA expressions of inflammatory factors interleukin-1β(IL-1β), interleukin-6(IL-6) and tumor necrosis factor(TNF-α) were significantly decreased(P<0.05 or P<0.01). The relative mRNA expression of liver apoptosis gene B lymphoblastoma/leukemia-2-associated X protein(Bax) significantly decreased(P<0.01), B lymphoblastoma 2 gene(Bcl-2) significantly increased(P<0.01), Bax/Bcl-2 mRNA expression significantly decreased(P<0.01). These results indicated that LYC could improve the antioxidant capacity of goslings and reduce the release of inflammatory factors by affecting Nrf2/HO-1/NQO1 signaling pathway, and at the same time affect the apoptosis index of Bcl-2/Bax, so as to alleviate the damage of liver tissue function and parenchyma caused by transport stress.
作者
杨昊天
王志强
苏景
冯国峰
黄宇翔
邹跃
马志刚
霍明东
张红
董佳强
吕明哲
张国华
刘雪松
王爽
沈思思
张艳
王欢
江波涛
钟鹏
陈志峰
YANG Haotian;WANG Zhiqiang;SU Jing;FENG Guofeng;HUANG Yuxiang;ZOU Yue;MA Zhigang;HUO Mingdong;ZHANG Hong;DONG Jiaqiang;LYU Mingzhe;ZHANG Guohua;LIU Xuesong;WANG Shuang;SHEN Sisi;ZHANG Yan;WANG Huan;JIANG Botao;ZHONG Peng;CHEN Zhifeng(Animal Husbandry and Veterinary Branch of Heilongjiang Academy of Agricultural Sciences,Qiaihar 161005,China;Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine Northeast Agricultural University,Harbin 150030,China;Heilongjiang Animal Disease Prevention and Control Center,Harbin 150069,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2024年第15期6-13,117,共9页
Heilongjiang Animal Science And veterinary Medicine
基金
国家现代农业产业技术体系项目(CARS-42-34)
黑龙江省农业科学院畜牧兽医分院自拟课题项目(ZNKT-202216)。
关键词
运输应激
番茄红素
氧化应激
凋亡
籽鹅
肝脏
transport stress
lycopene
oxidative stress
apoptosis
goslings
liver
