摘要
为促进木麻黄生长并解决根际土壤微生物群落结构和功能异常问题,本研究从木麻黄根瘤中筛选出具备固氮(N)、产细胞壁降解酶(蛋白酶和纤维素酶)、产生长素(IAA)、产铁载体、产氨气(NH_(3))、溶解磷酸盐等多种功能的8株菌株,其中LB08、LB18、LB19、LB42、LB46、LB63和LB69为类芽孢杆菌,LQ10为布鲁氏菌。菌液浸种试验显示:8株菌株均对木麻黄幼苗生长具有促进效果,其中菌株LB69显著提高了木麻黄种子萌发率和幼苗活力,增幅分别达19.7%和28.3%;菌株LQ10显著提升了根长和根系活力,增幅分别为48.2%和334.4%;菌株LB18和LB42分别对初期芽长和生物量积累具有最显著的促进效果,增长率分别为22.4%和32.8%。此外,浸种后幼苗多酚氧化酶、超氧化物歧化酶、过氧化物酶等的同工酶谱条带数增多,个别条带增强,酶亚型多样性增加,抗逆能力增强。综上,本文所述8株菌株的添加对植物生长及抗氧化酶活性具有促进效果,有成为生物肥料的潜力。
To promote the growth of Casuarina equisetifolia and address the abnormalities in the structure and function of rhizosphere soil microbial community, we isolated eight strains with multiple functions from the root nodules of C. equisetifolia, including nitrogen fixation(N), production of cell wall-degrading enzymes(protease and cellulase), auxin(IAA) production, siderophore production, ammonia(NH_(3)) production, and phosphate solubilization. Among these strains, LB08, LB18, LB19, LB42, LB46, LB63, and LB69 were identified as Paenibacillus species, while LQ10 was identified as a Brucella sp. Results of seed soaking experiments showed that all the eight strains promoted the growth of C. equisetifolia seedlings. Strain LB69 significantly increased the germination rate and seedling vigor by 19.7% and 28.3%, respectively. Strain LQ10 significantly enhanced root length and root vigor by 48.2% and 334.4%, respectively. Strains LB18 and LB42 had the strongest effects on early shoot length and biomass accumulation, with increases of 22.4% and 32.8%, respectively. After seed soaking, the number of isozymes bands of polyphenol oxidase, superoxide dismutase, and peroxidase increased, with some bands showing enhanced intensity and increased diversity of enzyme isoforms, thereby enhancing stress resistance. In summary, the addition of these eight strains promoted plant growth and antioxidant enzyme activity, indicating their potential role as biofertilizers.
作者
储薇
王炎炎
郭玥
彭艳晖
吴则焰
林文雄
CHU Wei;WANG Yanyan;GUO Yue;PENG Yanhui;WU Zeyan;LIN Wenxiong(College of JunCao Science and Ecology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《应用生态学报》
CAS
CSCD
北大核心
2024年第8期2159-2166,共8页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(31500443)
福建省自然科学基金项目(2022J01139)
福建省财政林业科技研究项目(KLY23002XA)
福建农林大学科技创新专项(KFb22046XA,KJb22019XA)
福建农林大学生态学一流学科建设项目资助。
关键词
木麻黄
促生
根际微生物
同工酶
Casuarina equisetifolia
plant growth promotion
rhizobacteria
isozyme
