摘要
为探究黄精轮作后对三七连作土壤微生物群落的影响,评估轮作及自然休耕两种方式的修复能力。本研究以三七二年连作土壤为对象,在三七连作后种植二年黄精,并以同等条件土壤休耕后再复种三七,基于微生物组学结合生信分析技术研究黄精轮作对三七连作土壤微生物结构组成及菌群变化的影响。结果显示:黄精轮作后复种三七一年存苗率可达88.33%,显著高于休耕处理;三七根腐发病率均低于20%,但相较于休耕处理差异不显著。黄精轮作后土壤真菌及细菌多样性均显著降低(P<0.01),主坐标分析结果表明:轮作能显著影响三七连作土壤真菌及细菌β多样性(P<0.001);轮作能显著增加子囊菌门相对丰度,降低担子菌门、被孢霉门真菌相对丰度,轮作后细菌厚壁菌门、芽单胞菌门相对丰度显著增加,绿弯菌门、不动杆菌门、拟杆菌门等显著降低。LEfSe结果表明,黄精轮作后三七根腐病病原菌镰刀菌属、Ilyonectria等真菌显著降低;芽孢杆菌属、芽单胞菌属、溶杆菌属、类芽胞杆菌属等重要细菌属显著增加。根际土壤微生物真菌主要以腐生、病理以及混合营养模式为主,经黄精轮作处理后,植物病原物真菌丰度下降,致病菌的占比有所下调,与病害有关的微生物下降,同时黄精轮作后细菌L-缬氨酸生物合成途径、L-异亮氨酸生物合成I(来自苏氨酸)途径、L-异亮氨酸生物合成II、有氧呼吸I(细胞色素C)途径显著降低。利用黄精轮作能够改善土壤微生物,对缓解三七连作障碍具有一定潜力。
In order to explore the influence of Polygonatum sibiricum rotation on the soil microbial community of Panax notoginseng,evaluate the restoration ability of crop rotation and natural restoration.The influence of Polygonatum sibiricum rotation on the microbial structure and microflora change of soil continuous cropping with Panax notoginseng was studied.The results showed that seedling rate was 88.33%,significantly higher than the incidence rate of fallow treatment,which was less than 20%,but the difference was not significant.The diversity of soil fungi and bacteria was significantly reduced(P<0.01).The results showed that crop rotation could significantly affect theβdiversity of soil fungi and bacteria in Panax notoginseng(P<0.001),and could significantly increase the relative abundance of Ascomycota and reduce the relative abundance of Basidiomycota and Triguta.Firmicutes,Budlesomonas,Chloritans,Acinetobacter,Bacteroidetes,etc.LEfSe results showed that after the rotation of the pathogens,Fusarium,Ilyonectria,and other fungi.Rhizosphere soil microbial fungi mainly saprophytic,pathological and mixed nutrition mode,after the rotation treatment,plant pathogen fungi abundance,pathogenic bacteria of decline,associated with the disease,at the same time after rotation bacterial L-valine biosynthesis pathway,L-isoleucine biosynthesis I(from threonine)pathway,L-isoleucine biosynthesis II,Aerobic respiration I(cytochrome C)pathway is significantly lower.The rotation of Polygonatum sibiricum can improve soil microorganisms and it's potential to alleviate the obstacles of Panax notoginseng.
作者
陈子涵
何涛
施本义
李科
蔡红
何霞红
朱有勇
赵宇
CHEN Zihan;HE Tao;SHI Benyi;LI Ke;CAI Hong;HE Xiahong;ZHU Youyong;ZHAO Yu(National Engineering Research Center for Agricultural Biodiversity Application Technology,Yunnan Agricultural University,Key Laboratory of Agricultural Biodiversity and Pest Control,Ministry of Education,Kunming 650201,China;College of Landscape and Horticulture,Southwest Forestry University,Kunming 650224,China;Lancang Liangbao Biotechnology Co.,Ltd,Puer 665000,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2024年第4期787-798,共12页
Acta Phytopathologica Sinica
基金
国家重点研发计划(2021YFD1000204)
云南省重大科技专项(202002AE90140)
澜沧县经济作物良种繁育技术集成与示范(202002AE320004)。
关键词
三七
黄精
轮作
土壤微生物
连作障碍
Panax notoginseng
Polygonatum sibiricum
crop rotation
soil microorganism
continuous obstacles
