摘要
邻苯二甲酸酯(PAEs)因具有改善塑料柔软性和柔韧性的特性而被作为增塑剂广泛应用于各种食品包装材料,然而,其对人类健康的重大危害使得建立准确的分析方法显得至关重要。为了解决油性基质样品前处理费时费力的问题,我们开发了一种基于顶空固相微萃取(HS-SPME)结合气相色谱-质谱(GC-MS)检测的分析方法,用于快速检测植物油基质中的12种PAEs。将苄基化环糊精超交联聚合物(BnCD-HCP)涂敷在不锈钢纤维上制备了一种具有较大比表面积、良好稳定性好和较高重复性的固相微萃取探针,并对其结构、形貌进行了表征。将油脂样品以甲醇改性后以该探针顶空萃取PAEs,优化得到如下最佳萃取参数:萃取时间20 min,萃取温度50℃,解吸时间4 min,解吸温度275℃。在优化的分析条件下,12种目标PAEs在线性范围内具有良好的线性关系(相关系数(R^(2))均大于099),检出限和定量限分别为021~374μg/kg和069~1234μg/kg,单针的相对标准偏差(RSD)≤114%,针间RSD≤139%。将此方法应用于大豆油、花生油和葵花油样品中PAEs的检测,加标试验结果表明该方法具有良好的精密度(RSD为117%~1173)和回收率(7249%~12443%)。该研究建立的基于BnCD-HCP的HS-SPME方法不需要溶剂萃取和净化等繁琐操作,具有快速、灵敏、准确、环保等优点,为植物油中PAEs的灵敏筛检提供了新的方法和技术。
Phthalate esters(PAEs)are used as additives to enhance the pliability and mallea-bility of plastics.These substances frequently migrate from packaging materials to vegetable oils because of the absence of covalent bonds.Over time,this migration could result in the accumu-lation of PAEs in the human body through ingestion,contributing to various diseases.There-fore,accurate qualitative and quantitative analyses of PAEs in vegetable oils are imperative to assess the origins of contamination and investigate their toxicity,degradation,migration,and transformation patterns.However,the concentration of PAEs in most samples is low,and the composition of vegetable oils is complex.Thus,PAEs must be enriched and purified using appropriate sample pretreatment procedures before analysis.Common methods for pretreating PAEs in oil include solid-phase extraction(SPE),dispersive SPE,and magnetic SPE.These techniques require time-consuming and labor-intensive procedures such as oil dissolution,sol-vent extraction,and degreasing.These approaches also require numerous solvents and contain-ers,increasing the risk of sample cross-contamination.Solid-phase microextraction(SPME)integrates sampling,extraction,purification,concentration,and injection into a single process,significantly accelerating analytical testing and reducing the potential for sample cross-contamination.In headspace(HS)mode,the analytes achieve equilibrium on the coating and are extracted in the gas phase.The fibers are shielded from nonvolatile and high-relative molec-ular mass substances in the sample matrix.Thus,SPME is an ideal method for extracting vola-tile compounds in vegetable oils.When HS-SPME coupled with gas chromatography-mass spec-trometry(GC-MS),it can achieve the rapid screening of PAEs in vegetable oil.In this study,an SPME with cyclodextrin-based hypercrosslinked polymers(BnCD-HCP)coated on stainless steel fibers was employed to extract PAEs from vegetable oil.The structure and morphology of the polymers were characterized using Fourier-transform infrared spectros-copy,nuclear magnetic spectroscopy,and scanning electron microscopy.BnCD-HCP exhibited high stability and diverse interactions,includingπ-π,hydrophobic,and host-guest interactions.The oil samples were incubated with methanol,and the PAEs were extracted from the head-space using the probe.The optimal extraction parameters included an extraction time of 20 min,extraction temperature of 50℃,desorption time of 4 min,and desorption temperature of 275℃.The BnCD-HCP/HS-SPME method was evaluated under optimized experimental condi-tions.The limits of detection(LODs)and quantification(LOQs)were determined by applying signal-to-noise ratios(S/N)of 3 and 10,respectively.Method accuracy was evaluated using relative standard deviations(RSDs).Single-needle precision was evaluated by conducting three consecutive analyses at 3 h intervals within a day.Inter-needle precision was assessed by con-ducting the same analyses(three replicates)with differently coated fibers.The 12 PAE com-pounds exhibited good linearity with correlation coefficients(R^(2))of at least 0.99.The LODs and LOQs ranged from 0.21 to 3.74μg/kg and from 0.69 to 12.34μg/kg,respectively.The RSDs were in the range of 1.8%-11.4%and 5.1%-13.9%for the single-needle and needle-to-needle methods,respectively.The proposed method was applied to soybean,peanut,and sun-flower oils,and two PAEs were found in all three oils.Moreover,the method demonstrated good precision(RSD=1.17%-11.73%)and recoveries(72.49%-124.43%).Compared with other methods,the developed method was able to extract many target analytes and had a low or comparable LOD and high recovery.More importantly,this method does not require tedious operations such as solvent extraction and purification.Consequently,the developed method can be used to extract not only PAEs in oils but also other substances with a high lipid content.
作者
张朋成
王媛
刘坤玲
孙亚明
何丽君
赵文杰
ZHANG Pengcheng;WANG Yuan;LIU Kunling;SUN Yaming;HE Lijun;ZHAO Wenjie(School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001,China;Ningxia Academy of Metrology and Quality Inspection,Yinchuan 750411,China)
出处
《色谱》
CAS
CSCD
北大核心
2024年第9期881-890,共10页
Chinese Journal of Chromatography
基金
国家自然科学基金(22176050)
河南省高校创新人才资助项目(20HASTIT007).
关键词
环糊精超交联聚合物
顶空固相微萃取
气相色谱-质谱
邻苯二甲酸酯
植物油
cyclodextrin-based hypercrosslinked polymers
headspace solid-phase microex-traction(HS-SPME)
gas chromatography-mass spectrometry(GC-MS)
phthalate esters
veg-etable oils
