摘要
地衣芽胞杆菌(Bacillus licheniformis)是微生物发酵饲料常用菌。本研究旨在通过优化发酵过程中最重要的碳源因子来提高地衣芽胞杆菌菌株ACCC 01957的菌体量和芽胞产量。首先基于ACCC 01957对Biolog GenⅢ 板的71种碳源进行利用效率筛选,再采用全自动细胞生长曲线仪分析优势碳源对生物量、生长周期及产孢率的影响,并在摇瓶水平上确定最佳发酵碳源。结果表明,菌株ACCC 01957可以较好地利用25种碳源,其中N-乙酰-β-D-甘露糖胺、D-半乳糖、L-鼠李糖、肌苷、甘油、果胶、L-乳酸、吐温-40、L-天冬氨酸、L-谷氨酸和L-焦谷氨酸可以促进其生长和产孢,而D-山梨醇促生长抑产孢;在摇瓶水平上以葡萄糖和L-鼠李糖为复合碳源(添加比例为16)时,培养24 h的菌体总量为8.3×10^(8) cfu/mL,培养48 h的产孢产率高达91.19%。通过产芽胞碳源组合构建提高了地衣芽胞杆菌ACCC 01957的生长、产芽胞率和产芽胞量。
Bacillus licheniformis is commonly used in fermented feed.This experiment aims to improve the biomass and spore yield of B.licheniformis strain ACCC 01957 by optimizing the most important factor,carbon source,in the fermentation process.The utilization efficiency of 71 carbon sources on Biolog GenⅢ plate was firstly screened,the effect of favorable carbon sources on the biomass,growth cycle and sporulation rate was then analyzed by using an automatic cell growth analyzer,and the optimal fermentation conditions were determined on the flask level.The results showed that ACCC 01957 made better use of 25 carbon sources,and N-acetyl-β-D-mannosamine,D-galactose,L-rhamnose,inosine,glycerin,pectin,L-lactic acid,Tween 40,L-aspartic acid,L-glutamic acid and L-pyroglutamic acid simultaneously promoted the growth and sporulation;sorbitol was identified as the carbon source for promoting growth and inhibiting spore production;when glucose and L-rhamnose were combined at the ratio of 16 in shaking flask level,the biomass at 24 h was the highest(8.3×10^(8) cfu/mL),and the sporulation rate at 48 h reached the maximum(91.19%).This study greatly improved the growth,spore yielding and sporulation rate of B.licheniformis ACCC 01957 by optimizing the carbon source.
作者
马聪聪
马锐
王云山
宋国彪
顾金刚
MA Cong-cong;MA Rui;WANG Yun-shan;SONG Guo-biao;GU Jin-gang(Inst.Agric.Resour.Reg.Plan.,Chin.Acad.Agric.Sci.,Beijing 100081;Inst.Proc.Eng.,Chin.Acad.Sci.,Beijing 100190;Han′en Biol.Technol.Ltd.Co.,Tongliao 028000)
出处
《微生物学杂志》
CAS
CSCD
北大核心
2024年第4期40-47,共8页
Journal of Microbiology
基金
内蒙古通辽市科技成果转化项目(TLZH2021003)。
关键词
地衣芽胞杆菌
菌体量
产芽胞率
碳源优化
Bacillus licheniformis
bacterial mass
sporulation rate
carbon source optimization
