摘要
腐殖酸生物降解地膜是一种新型生物降解地膜,因其覆盖栽培番茄能显著改良土壤环境,提高果实品质而受到广泛关注。为探明其对番茄果实品质和营养成分的影响及其调控机制,以设施番茄‘甜脆脆’为试验材料,对PBAT/PLA普通生物降解膜(PT)和添加腐殖酸的生物降解膜(FZS)处理下的番茄成熟果实进行风味感官评价,并测定可溶性糖、番茄红素等营养物质含量,利用PCA、正交偏最小二乘法、KEGG等分析方法对成熟果实转录组和GC/LC–MS非靶向代谢组进行联合分析。结果表明:与PT处理成熟果实相比,FZS处理果实薄壁细胞数显著减少28.10%,表皮层和角质层厚度分别减少24.28%和43.99%,促进了果实的成熟,并提高了可溶性糖和糖酸比,降低了酸度和硬度,促进了果实的成熟,改善了品质。转录组分析共鉴定出500个差异表达基因,主要富集于碳水化合物代谢、糖酵解和细胞壁结构等途径。代谢组分析共鉴定出143个差异含量代谢物,主要富集于丁酸代谢、赖氨酸降解和ABC转运蛋白等途径。转录—代谢组联合分析发现FZS对番茄果实风味品质的影响受到碳水化合物/能量代谢以及氨基酸和激素转运等途径的正向调控,其中葡萄糖–6–磷酸、果糖–6–磷酸和半乳糖参与的碳水化合物代谢通路是主要的调控途径。
Humic acid biodegradable plastic film is a new type of biodegradable plastic film that has received widespread attention due to its significant improvement in the soil quality of facility cultivation and the increase in tomato quality.To investigate the effects and regulatory mechanisms of humic acid biodegradable plastic film on the quality and nutritional components of tomato fruits,this study used facility tomato‘Tiancuicui’as the experimental material,flavor sensory evaluation was conducted on mature tomato fruits treated with PBAT/PLA ordinary degradable plastic film(PT)and humic acid biodegradable plastic film(FZS),and the content of soluble sugars,lycopene and other nutrients were measured.PCA,OPLS-DA,KEGG and other analysis methods were used to jointly analyze the transcriptome and GC/LC–MS non targeted metabolomic of ripe fruits.The results showed that FZS treatment significantly reduced the number of thin-walled cells by 28.10%,thickness of epidermis and stratum corneum in tomato fruit by 24.28%and 43.99%,respectively,promoting tomato fruit ripening,increasing soluble sugar and sugar acid ratio,reducing fruit acidity and hardness,and improving fruit quality;transcriptome analysis identified 500 differentially expressed genes,mainly enriched in pathways such as carbohydrate metabolism,glycolysis,and cell wall structure;metabolomic analysis identified 143 differential metabolites,mainly enriched in pathways including butyric acid metabolism,lysine degradation,and ABC transporters;transcription metabolism joint analysis revealed that the impact of FZS on tomato fruit flavor quality is positively regulated by the carbohydrate/energy metabolism pathway,amino acid and hormone transport;the carbohydrate metabolism pathway involved in glucose-6-phosphate,fructose-6-phosphate,and galactose are the main regulatory pathways.
作者
韩荧
段颖
牛一杰
李衍素
贺超兴
孙敏涛
王君
李强
陈双臣
闫妍
HAN Ying;DUAN Ying;NIU Yijie;LI Yansu;HE Chaoxing;SUN Mintao;WANG Jun;LI Qiang;CHEN Shuangchen;YAN Yan(College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang,Henan 471000,China;Institute of Vegetables and Flowers Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Engineering,Horticultural and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2024年第8期1758-1772,共15页
Acta Horticulturae Sinica
基金
国家重点研发项目(2021YFD1600303,2022YFD1601505)
国家自然科学基金项目(32372793)
蔬菜生物育种全国重点实验室项目。
关键词
番茄
腐殖酸
生物可降解地膜
转录—代谢组联合分析
tomato
humic acid
biodegradable plastic film
transcription metabolomics combined analysis
