摘要
以2023年4月北京通州区3所幼儿园出现流感样病例症状(发热、咳嗽、咽痛、流鼻涕等呼吸道症状)者为研究对象,采集鼻咽拭子标本,采用实时荧光定量PCR方法对采集的标本进行常见呼吸道病原体检测,对阳性标本进行人呼吸道合胞病毒(HRSV)G蛋白高变区序列测序、同源性分析和构建进化树分析。3所幼儿园共收集发热病例25例,年龄为3~8岁,年龄M(Q_(1),Q_(3))为4(3.5,5)岁。应用荧光定量PCR方法筛查检出10例HRSV阳性确诊病例,总检出率40%(10/25)。分型鉴定及测序分析确认主要流行型别为B亚型HRSV,且与该地区既往流行株高度同源,亲缘关系近。通过病原学调查分析,初步判断此次疫情是以B亚型HRSV为主导流行型别的疫情。
The study focused on individuals with influenza-like symptoms(fever,cough,sore throat,runny nose,and other respiratory symptoms)in three kindergartens in Tongzhou District,Beijing City,in April 2023.Nasopharyngeal swab specimens were collected,and real-time fluorescent quantitative PCR was used to detect common respiratory pathogens in the collected specimens.Positive specimens were subjected to sequencing analysis of the highly variable region of human respiratory syncytial virus(HRSV)G protein,homology analysis and phylogenetic tree analysis.A total of 25 fever cases were collected from 3 kindergartens,aged 3-8 years old,with an age M(Q_(1),Q_(3))of 4(3.5,5)years old.Ten confirmed cases of HRSV positive were screened and detected using the fluorescent quantitative PCR method,with a total detection rate of 40%(10/25).Typing identification and sequencing analysis confirmed that the main epidemic type was HRSV subtype B,which was highly homologous and closely related to previous epidemic strains in the region.Through pathogen investigation and analysis,it was preliminarily determined that this epidemic was dominated by HRSV subtype B.
作者
邹林
张冲
佟玲
刘肖
马静
王建国
王芳
高翔
郗璐
张建明
Zou Lin;Zhang Chong;Tong Ling;Liu Xiao;Ma Jing;Wang Jianguo;Wang Fang;Gao Xiang;Xi Lu;Zhang Jianming(Beijing Tongzhou District Center for Disease Control and Prevention,Beijing 101100,China)
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2024年第8期1150-1153,共4页
Chinese Journal of Preventive Medicine
基金
“十三五”国家重大专项(2017ZX10103004-002)
首发专项(2022-1G-3014)。
关键词
人呼吸道合胞病毒
儿童
基因分型
病原学
Human respiratory syncytial virus
Children
Genotyping
Etiology
