摘要
基于视网膜变性(retinal degeneration diseases,RDD)小鼠模型,采用多元统计分析方法比较不同产地枸杞子的视网膜保护作用。通过腹腔注射碘酸钠(NaIO_(3))构建小鼠RDD小鼠模型,采用双门黑白箱和TUNEL测定评估视觉功能和视网膜细胞凋亡情况;采用眼底光学相干断层扫描(OCT)检测视网膜厚度并进行眼底照相,测定血清和眼球中氧化应激、炎症和血管生成标志物的水平。采用层次聚类分析、主成分分析和偏最小二乘法判别分析进行不同产地枸杞子对小鼠RDD模型视网膜保护作用的综合比较。结果显示,不同产地枸杞子提取物均可逆转NaIO_(3)诱导的视觉及视网膜损伤和细胞凋亡,改善小鼠体内氧化应激水平及炎症因子和血管生成标志物的表达。基于17个药效学指标进行多元统计分析后发现,产于宁夏的枸杞子提取物相较于其余4个产地的受试样品呈现更好的RDD治疗效应。研究结果可为防治RDD的枸杞子优势产地选择提供科学依据。
The multivariate statistical analysis was performed to compare the therapeutic effects of Lycii Fructus from different origins on the retinal degenerative diseases(RDD)in mice.The mouse model of RDD was established by intraperitoneal injection of NaIO_(3),and the visual function and retinal apoptosis were assessed by dark-light transition and TUNEL assay.Retinal thickness was measured by fundus optical coherence tomography(OCT),and the levels of antioxidant,inflammatory,and angiogenic markers in the serum and eyeball were determined.The therapeutic effects were compared by hierarchical cluster analysis,principal component analysis,and partial least squares-discriminant analysis.The results showed that the extracts of Lycii Fructus from different origins reversed NaIO_(3)-induced visual damage and retinal apoptosis,reduced oxidative stress,and restored the expression of inflammatory mediators and angiogenic markers in mice.The multivariate statistical analysis based on 17 pharmacodynamic indices suggested that the extract of Lycii Fructus from Ningxia demonstrated better therapeutic effects on RDD than the samples from the other four origins.The results of this study provide a scientific basis for the selection of the advantageous production region of Lycii Fructus for the prevention and treatment of RDD.
作者
陈鑫
郭盛
王杰美
李洁
倪亮
康宏杰
钱大玮
段金廒
CHEN Xin;GUO Sheng;WANG Jie-mei;LI Jie;NI Liang;KANG Hong-jie;QIAN Da-wei;DUAN Jin-ao(National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine/Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/National Administration of Traditional Chinese Medicine Key Laboratory for Chinese Medicine Resources Recycling Utilization,Nanjing University of Chinese Medicine,Nanjing 210023,China;Afiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Ningxia Innovation Center of Goji R&D,Yinchuan 750002,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第16期4521-4531,共11页
China Journal of Chinese Materia Medica
基金
宁夏重点研发计划重点项目(2021BEF02010,2021BEF02009)
国家自然科学基金区域创新发展联合基金重点项目(U21A20408)
江苏省重点研发计划项目(BE2021907,BE2021912)
中央本级重大增减支项目(2060302)。
关键词
枸杞子
视神经保护
视网膜变性
多元统计分析
Lycii Fructus
optic nerve protection
retinal degenerative disease
multivariate statistical analysis
