摘要
为了建立矾根组培快繁体系,以矾根去顶尖幼嫩顶芽为外植体,研究不同培养基对胚性愈伤组织诱导、胚性愈伤组织增殖以及植株再生的影响。结果表明,愈伤组织最佳培养基为MS+2.4-D 3.0 mg/L+6-BA 0.3 mg/L,愈伤诱导率达到93.0%,团块较大,结构较松散,有颗粒状态,个别团块呈水浸状;以最优胚性愈伤组织增殖培养基为MS+2.4-D 2.0 mg/L+6-BA 0.2 mg/L进行扩增,愈伤组织发育较好,结构较松散,颗粒状疣突分明,水分较少,呈淡黄绿色。胚状体分化最佳培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+IBA 0.2 mg/L,胚状体诱导率达91.8%。对幼根发育不良的植株,在1/2MS+IBA 0.1 mg/L+IAA 0.1 mg/L培养基上进行生根培养效果好,生根率100%,平均根数5.5条,根长2~3 cm,苗高4.1 cm。
In order to establish tissue culture and rapid propagation system of Heuchera micrantha,using the young terminal buds of Heuchera micrantha as explants,this study investigated the effects of different media on the induction,proliferation,and regeneration of embryogenic callus tissues.The results showed that the medium MS+2.4-D 3.0 mg/L+6-BA 0.3 mg/L had a high callus induction rate of 93.0%.The callus formed was large,loosely structured,and grainy but immature,with some clusters appearing water-soaked.For the proliferation of embryogenic callus tissues,the medium MS+2.4-D 2.0 mg/L+6-BA 0.2 mg/L resulted in well-developed callus tissues that were loosely structured with distinct granular nodules and less water content,presenting a light yellow-green color.The differentiation medium MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+IBA 0.2 mg/L achieved a differentiation rate of 91.8%.For plants with poorly developed young roots,rooting culture on 1/2 MS+IBA 0.1 mg/L+IAA 0.1 mg/L resulted in a rooting rate of 100%,with an average of 5.5 roots per plant,root lengths of 2 to 3 cm,and plant height of 4.1 cm.
作者
任俞新
REN Yuxin(Gansu Forestry Professional Technology College,Tianshui Gansu 741020,China)
出处
《寒旱农业科学》
2024年第8期747-751,共5页
Journal of Cold-Arid Agricultural Sciences
关键词
矾根
去顶尖幼嫩顶芽
胚性愈伤组织
诱导
植株再生
Heuchera micrantha
Terminal bud
Embryogenic callus
Induction
Plant regeneration
