摘要
为评估不同种鸟类的病原体传播风险,提取红嘴鸥和喜鹊粪便的总DNA,通过PCR扩增16S rRNA序列V3~V4高变区,基于高通量测序和生物信息学研究两者肠道微生物群差异。研究结果显示,红嘴鸥和喜鹊粪便微生物群组成存在显著差异,两者粪便中最丰富的细菌门分别是厚壁菌门和变形菌门,前者粪便中绝对优势属是Catellicoccus(丰度均值65.5%)。红嘴鸥样本中均检测出弯曲杆菌属,检出率100%,丰度均值1.2%;喜鹊样本检出率55.6%,丰度均值0.019%。这表明,青岛市红嘴鸥种群广泛存在弯曲杆菌属,红嘴鸥的接触安全风险高于喜鹊。
Total DNA from fecal samples were extracted and the V3~V4 hypervariable region of the 16S rRNA sequence was amplified to assess the potential risk of bird-to-human pathogen transmission in different bird species.Gut microbiota characterizations were determined with high-throughput sequencing and the followed bioinformatical analyses.The results show remarkable differences in the fecal microbiota between black-headed gulls and magpies.Firmicutes and Proteobacteria are the primary bacterial phyla in black-headed gull and magpie feces,respectively.Catellicoccus is the predominant genus in black-headed gull feces,and the average abundance is 65.5%.The detective rate of Campylobacter in black-headed gull feces is 100%,and the average abundance is 1.2%.But in magpie feces,the detective rate is 55.6%,and the average abundance is 0.019%.This suggests that Campylobacter is widespread in the population of black-headed gull in Qingdao,and the safety risk of exposure to black-headed gulls is higher than magpies.
作者
刘文轩
杨笑礼
李宗会
王斌
LIU Wen-xuan;YANG Xiao-li;LI Zong-hui;WANG Bin(School of Basic Medicine,Qingdao University,Qingdao 266000,China)
出处
《青岛大学学报（自然科学版）》
CAS
2024年第3期15-20,26,共7页
Journal of Qingdao University(Natural Science Edition)
基金
国家重点研发计划(批准号:2018YFA0900802)资助
山东省重点研发计划(批准号:2019JZZY011009)资助
青岛市科技计划重点研发专项(批准号:20-2-3-4-nsh)资助。
关键词
红嘴鸥
喜鹊
肠道菌群
高通量测序
black-headed gull
magpie
intestinal microbiota
high-throughput sequencing
