摘要
研究旨在优化益母草碱脂质体制备工艺,以制备包封率较高、粒径较小的益母草碱脂质体。试验以益母草碱包封率、粒径、多分散性指数(PDI)为评价指标,筛选最适合的益母草碱脂质体制备方法;之后以包封率为评价指标比较药脂比、胆磷比、PBS溶液体积、超声时间、表面活性剂浓度对益母草碱脂质体包封率的影响,筛选影响更显著的因素进行响应面试验,并以得到的最佳条件进行验证试验。结果显示,薄膜分散法结合pH值梯度法制备益母草碱脂质体的最佳药脂比为1∶15.84,胆磷比为1∶13.4,PBS体积为9.92 mL,该条件下制备的益母草碱脂质体包封率为92.18%,平均粒径为56.01 nm,PDI为0.095。研究表明,薄膜分散法结合pH值梯度法制备益母草碱脂质体包封率高,符合试验要求。
The study aimed to optimize the preparation process of leonurine liposomes to achieve high encapsulation efficiency and small particle size.The trial evaluated the encapsulation efficiency,particle size,and polydispersity index(PDI)as indicators to select the most suitable method for preparing leonurine liposomes.Subsequently,the encapsulation efficiency was used as the evaluation criterion to compare the effects of the drug-to-lipid ratio,cholesterol-to-phospholipid ratio,PBS solution volume,ultrasonication time,and surfactant concentration on the encapsulation efficiency of leonurine liposomes.The more significant factors affecting the encapsulation efficiency were identified for response surface testing,and the optimal conditions obtained were verified in a confirmation test.The results showed that the thin-film dispersion method combined with the pH gradient method prepared leonurine liposomes with the best drug-to-lipid ratio of 1∶15.84,cholesterol-to-phospholipid ratio of 1∶13.4,and PBS solution volume of 9.92 mL.Under these conditions,the encapsulation efficiency of leonurine liposomes was 92.18%,the average particle size was 56.01 nm,and the PDI was 0.095.The study indicates that the thin-film dispersion method combined with the pH gradient method for preparing leonurine liposomes has a high encapsulation rate,meeting the trial requirements.
作者
霍守雨
刘权辉
李卫清
黄奔
Huo Shouyu;Liu Quanhui;Li Weiqing;Huang Ben(Subtropical Agricultural Bioresources Protection and Utilization National Key Laboratory,Guangxi University,Guangxi Nanning 530004;Guangxi Zhuang Autonomous Region People's Hospital,Guangxi Nanning 530004)
出处
《现代畜牧兽医》
2024年第7期7-12,共6页
Modern Journal of Animal Husbandry and Veterinary Medicine
关键词
益母草碱
脂质体
响应面试验
包封率
Leonurine
Liposomes
Response surface methodology
Encapsulation efficiency
