摘要
条纹病是重要的种传真菌病害之一,能对青稞生产造成严重产量损失。为明确分布于青海的青稞条纹病原菌致病性差异及遗传多样性,本研究比较了分离自青海省不同地区18个条纹菌菌株形态特征差异,利用rDNA-ITS序列分析菌株间遗传多样性,同时测定了病菌致病性变异情况。青稞条纹病原菌于PDA培养基上菌落颜色多样,菌落质地的疏松度和边缘的规则程度都有一定的差异;在PDA培养基上不能产生分生孢子;菌株培养7 d后菌落直径范围为27.4~81.1 mm,菌株的生长速度存在显著差异。致病性分化结果显示,条纹病原菌株对青稞品种的致病性存在差异,可将18个测试菌株分成2个组,且致病性与病菌地区来源无明显相关性。依据系统进化树分析结果,在欧氏距离为9.58时将18个菌株分成2个亚组,说明条纹菌遗传多样性水平较低。菌株间亲缘关系与其来源地区无明显相关性,说明条纹病原菌在不同地区存在较多的交流。本研究能为青稞条纹病的防治和后续研究中菌株的选择提供理论依据。
Stripe disease is one of the important seed-borne fungal diseases,which can cause serious yield loss to hulless barley production.In order to clarify the pathogenic variation and genetic diversity of hulless barley stripe pathogens distributed in Qinghai,this study compared the morphological characteristics of 18 stripe isolates from different areas in Qinghai Province,analyzed the genetic diversity among isolates by rDNA-ITS sequence and determined the pathogenicity variation of the pathogens.The color characteristics of the colony of the hulless barley stripe pathogen on the PDA medium were variable,and the looseness of the colony texture and the regularity of the edge had certain differences.Conidia cannot be produced on PDA medium;the colony diameter of the isolate ranges from 27.4 to 81.1 mm after the strain was cultured for 7 days,there were significant differences in the growth rates of the isolates.The results of pathogenic differentiation showed that there were differences in the pathogenicity of striped pathogenic isolates to hulless barley varieties.The 18 tested strains could be divided into 2 groups,and the pathogenicity had no obvious correlation with the regional source of the pathogen.According to the phylogenetic tree analysis,the 18 strains were divided into 2 subgroups when the Euclidean distance was 9.58,indicating that the level of genetic diversity of striae was low.There was no obvious correlation between the phylogeny between isolates and their source regions,illustrating that stripe pathogens had more exchanges in different regions.This study can provide a theoretical basis for controlling barley stripe disease and the selection of isolates in subsequent studies.
作者
康胜华
赵珂
侯璐
Kang Shenghua;Zhao Ke;Hou Lu(Qinghai Provincial Key Laboratory of Integrated Agricultural Pest Management,Academy of Agriculture and Forestry Sciences,Qinghai University(Qinghai Academy of Agriculture and Forestry Sciences),Xining,810016)
出处
《分子植物育种》
CAS
北大核心
2024年第17期5725-5735,共11页
Molecular Plant Breeding
基金
国家自然科学基金项目(32160647)
青海省自然科学基金项目(2022-ZJ-909)
农业农村部农业种质资源普查收集保护鉴定服务项目(A120202)
青海省农业有害生物综合治理重点实验室项目(2022-ZJ-Y10)共同资助。
关键词
麦类核腔菌
青稞
条纹病
遗传多样性
致病性
Pyrenophora graminea
Hulless barley
Leaf stripe
Genetic diversity
Pathogenicity
