摘要
目的探讨血清miR-520e、miR-146a表达与老年乙型肝炎病毒(HBV)相关肝癌患者临床病理参数及预后的关系。方法将2018年2月—2021年2月本院收治的HBV相关肝细胞癌患者95例设为观察组,将同期HBV患者100例设为对照组,采用实时聚合酶链反应法(RT-PCR)检测两组血清中miR-520e和miR-146a水平。比较两组血清中miR-520e和miR-146a水平的差异,以及观察组中不同病理特征患者血清中miR-520e和miR-146a水平的差异,绘制不同miR-520e和miR-146a水平肝癌患者的1年无进展生存期生存曲线。结果观察组患者血清miR-520e、miR-146a水平均低于对照组患者(P<0.05);HCC患者中,病灶大小≥3 cm、有癌栓、伴淋巴结转移、TNM分期Ⅲ~Ⅳ期的患者血清miR-520e水平明显低于病灶大小<3 cm、无癌栓、不伴淋巴结转移、TNM分期Ⅰ~Ⅱ期的患者(P<0.05);HCC患者中,病灶大小≥3 cm、有癌栓、伴淋巴结转移、TNM分期Ⅲ~Ⅳ期的患者血清miR-146a水平明显低于病灶大小<3 cm、无癌栓、不伴淋巴结转移、TNM分期Ⅰ~Ⅱ期的患者(P<0.05);将观察组患者中miR-520e水平低于平均值患者分为低miR-520e表达组,高于平均值患者分为高miR-520e表达组;将观察组患者中miR-146a水平低于平均值患者分为低miR-146a表达组,高于平均值患者分为高miR-146a表达组。曲线分析结果显示,miR-520e和miR-146a高表达者1年内无进展生存期情况均明显优于低表达者(P<0.05)。结论HBV相关肝细胞癌患者中miR-520e和miR-146a血清水平显著低于HBV患者,且不同miR-520e和miR-146a血清水平的HBV相关肝细胞癌患者之间临床病理特征与预后存在显著差异。
Objective To explore the relationship between serum miR-520e,miR-146a and clinicopathological parameters,prognosis in elderly patients with hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC).Methods A total of 95 patients with HBV-related HCC and 100 patients with HBV in the hospital were enrolled as observation group and control group from February 2018 to February 2021.The levels of serum miR-520e and miR-146a in both groups were detected by real-time polymerase chain reaction(RT-PCR).The differences in the two indexes between the two groups and among patients with different pathological characteristics in observation group were compared.The 1-year progression-free survival curves in HCC patients with different levels of miR-520e and miR-146a were drawn.Results The levels of serum miR-520e and miR-146a in observation group were lower than those in control group(P<0.05).In HCC patients,levels of serum miR-520e and miR-146a in patients with lesion≥3cm,cancer thrombus,lymph node metastasis and TNM staging at stage III-IV were significantly lower than those with lesion<3cm,without cancer thrombus or lymph node metastasis,and with TNM staging at stageⅠ-Ⅱ(P<0.05).In observation group,patients with miR-520e level lower or higher than the average were enrolled as low-expression miR-520e group and high-expression miR-520e group.In observation group,patients with miR-146a level lower or higher than the average were enrolled as low-expression miR-146a group and high-expression miR-146a group.The results of curves analysis showed that 1-year progression-free survival in patients with high expressions of miR-520e and miR-146a was significantly better than that with low expressions(P<0.05).Conclusion The levels of serum miR-520e and miR-146a in patients with HBV-related HCC are significantly lower than those in HBV patients.In patients with HBV-related HCC,there are significant differences in clinicopathological characteristics and prognosis among patients with different levels of serum miR-520e and miR-146a.
作者
马慧敏
唐微
许丹
寇丹
宋辉
闫翔
MA Huimin;TANG Wei;XU Dan;KOU Dan;SONG Hui;YAN Xiang(Ward 1,Department of Cadre 2,Western Theater General Hospital,Chengdu 610083,China)
出处
《西部医学》
2024年第9期1336-1340,共5页
Medical Journal of West China
基金
四川省干部保健科研课题(川干研2021-1301)。
