摘要
Acellular dermal matrix(ADM)shows promise for cartilage regeneration and repair.However,an effective decellularization technique that removes cellular components while preserving the extracellular matrix,the transformation of 2D-ADM into a suitable 3D scaffold with porosity and the enhancement of bioactive and biomechanical properties in the 3D-ADM scaffold are yet to be fully addressed.In this study,we present an innovative decellularization method involving 0.125%trypsin and 0.5%SDS and a 1%Triton X-100 solution for preparing ADM and converting 2D-ADM into 3D-ADM scaffolds.These scaffolds exhibit favorable physicochemical properties,exceptional biocompatibility and significant potential for driving cartilage regeneration in vitro and in vivo.To further enhance the cartilage regeneration potential of 3D-ADM scaffolds.we incorporated porcine-derived small intestinal submucosa(SIS)for bioactivity and calcium sulfate hemihydrate(CSH)for biomechanical reinforcement.The resulting 3D-ADM+SIS scaffolds displayed heightened biological activity,while the 3D-ADM+CSH scaffolds notably bolstered biomechanical strength.Both scaffold types showed promise for cartilage regeneration and repair in vitro and in vivo,with considerable improvements observed in repairing cartilage defects within a rabbit articular cartilage model.In summary,this research introduces a versatile 3D-ADM scaffold with customizable bioactive and biomechanical properties,poised to revolutionize the field of cartilageregeneration.
基金
the National Natural Science Foundation of China(82302395,82102348,82001979,82372390 and 31900963)
the Natural Science Foundation of Shanghai(22YF1437400)
Young Elite Scientists Sponsorship Program by CAST(2023QNRC001)
the Health-Education Joint Research Project of Fujian Province(2019-WJ-22)
Taishan Scholar Program of Shandong Province(tsqn20230633)
ShanghiaHi ealth Promotion Commission,Shanghai 2023 Health Science Popularization Special Plan‘Prevention and Control Science Popularization System for Hip Fall Injury in the Elderly'(JKKPZX-2023-A27)and the Fundamental Research Funds for the Central Universities(2021CDJQY-017).
