摘要
目的研究黑果小檗内生真菌假丝酵母菌Candida sp.的次生代谢产物。方法假丝酵母菌发酵提取物次级代谢产物的乙酸乙酯部位、石油醚部位采用硅胶、Sephadex LH-20、制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到18个化合物,分别鉴定为1-苯基-1,2-乙二醇(1)、4-羟基苯乙醇(2)、4-羟基苯甲酸(3)、对羟基苯乙酸(4)、间羟基苯乙酸(5)、3-甲基亚砜基丙酸(6)、苯乙酸(7)、(S)-N-nitroso-1-amino-p-hydroxy phenylethanol(8)、2-苯乙酰胺(9)、对羟基苯甲醛(10)、2-(4-羟苯基)-乙酸乙酯(11)、邻苯二甲酸二丁酯(12)、5,5′-dimethoxybiphenyl-2,2′-diol(13)、3-吲哚甲醛(14)、N-乙酰-L-苯丙氨酸(15)、9-hydroxy-10 E,12Z-octadecadienoic acid(16)、9-hydroxy-10 E,12E-octadecadienoic acid(17)、(6E)-5-methylene-6-tetradecenoic acid(18)。结论化合物1、3~8、10~18为首次从假丝酵母菌真菌中分离得到。
AIM To study the secondary metabolites from the endophytic fungi Candida sp.of Berberis atrocarpa Schneid.METHODS The ethyl acetate fraction and petroleum ether fraction from the secondary metabolites of Candida sp.fermentation extract were separated and purified by silica gel,Sephadex LH-20 and preparative liquid chromatography,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Eighteen compounds were isolated and identified as 1-phenyl-1,2-ethanediol(1),4-hydroxyphenethyl alcohol(2),4-hydroxybenzoic acid(3),4-hydroxyphenylacetic acid(4),3-hydroxyphenylacetic acid(5),3-methylsulfinyl propionic acid(6),phenylacetic acid(7),(S)-N-nitroso-1-amino-p-hydroxy phenylethanol(8),2-phenylacetamide(9),p-hydroxybenzaldehyde(10),ethyl 2-(4-hydroxyphenyl)acetate(11),dibutyl phthalate(12),5,5′-dimethoxybiphenyl-2,2′-diol(13),3-indolealdehyde(14),N-acetyl-L-phenylalanine(15),9-hydroxy-10 E,12Z-octadecadienoic acid(16),9-hydroxy-10 E,12E-octadecadienoic acid(17),(6E)-5-methylene-6-tetradecenoic acid(18).CONCLUSION Compounds 1,3-8 and 10-18 are isolated from Candida sp for the first time.
作者
郭铭焯
麻淑芳
王世苗
冯亚萍
欧阳艳
庞克坚
焦子伟
木古丽木哈西
杨新洲
GUO Ming-zhuo;MA Shu-fang;WANG Shi-miao;FENG Ya-ping;OUYANG Yan;PANG Ke-jian;JIAO Zi-wei;MUHAXI Mu-gu-li;YANG Xin-zhou(Yili Normal University,Yining,Yining 835000,China;School of Pharmaceutical Sciences,South-Central Minzu University,Wuhan 430074,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第9期3000-3005,共6页
Chinese Traditional Patent Medicine
基金
新疆维吾尔自治区高校科研计划(XJEDU2018I018)。
关键词
黑果小檗
假丝酵母菌
内生真菌
次生代谢产物
分离鉴定
Berberis atrocarpa Schneid.
Candida sp.
endophytic fungi
secondary metabolites
isolation and identification
