摘要
生长素响应因子(ARFs)是调控植物各器官发育的重要转录因子,TaARF14是小麦ARF基因家族的成员之一.以小麦栽培品种‘尚农6’为材料,克隆了TaARF14基因(TaARF14-1A,TaARF14-1B和TaARF14-1D).序列分析显示:TaARF14的3个同源基因都具有10个外显子区域,且3条核酸序列相似性为94.94%~96.07%;TaARF14基因的启动子区域含有大量与植物正常生长发育或植物激素相关的顺式作用元件,推测TaARF14-1A,TaARF14-1B和TaARF14-1D对小麦的生长发育具有重要作用,且TaARF14基因对生长素(IAA)、赤霉素(GA)和脱落酸(ABA)具有明显的响应;系统进化分析表明:相比其他作物,ARF14基因在麦类作物基因进化中具有更高的保守性;qRT-PCR结果表明:TaARF14基因在小麦的穗发育进程中,特别是花器官的发育(包括雄蕊和雌蕊)中具有极其重要的作用,在施用IAA,GA和ABA后,TaARF14基因在根、茎、叶中均具有明显的响应变化.
Auxin response factors(ARFs) are important transcription factors that regulate the development of plant organs.TaARF14 gene is a member of wheat ARF gene family.In this study,TaARF14 genes(TaARF14-1A,TaARF14-1B and TaARF14-1D) were cloned from wheat cultivar Shangnong 6.The sequence analysis showed that the three homologous genes of TaARF14 had 10 exon regions,and the similarity of three nucleic acid sequences was between 94.94%~96.07%.The promoter region of TaARF14 genes contained a large number of cis-acting elements related to normal plant growth and development or plant hormones,suggesting that TaARF14-1A,TaARF14-1B and TaARF14-1D played an important role in the growth and development of wheat.TaARF14 genes had obvious response to IAA,GA and ABA.Phylogenetic analysis showed that ARF14 gene was more conserved in triticeae crops than in other crops.qRT-PCR results showed that TaARF14 gene played an extremely important role in the spike development process of wheat,especially in the development of flower organs(including stamen and pistil).After application of IAA,GA and ABA,expressions of TaARF14 genes showed significant changes in root,stem and leaf.This study will provide important reference for further study of TaARF14 gene function.
作者
连少英
张修引
郭灿
牛吉山
姜晓君
李磊
张庆琛
齐学礼
倪永静
LIAN Shaoying;ZHANG Xiuyin;GUO Can;NIU Jishan;JIANG Xiaojun;LI Lei;ZHANG Qingchen;QI Xueli;NI Yongjing(Shangqiu Academy of Agricultural and Forestry Sciences/Henan Engineering Research Center of Wheat Spring Freeze Injury Identification,Shangqiu Henan 476000,China;Suihuang Seed Industry Laboratory,Shangqiu Henan 476000,China;National Engineering Research Center for Wheat,Henan Agricultural University/Henan Technology Innovation Centre of Wheat,Zhengzhou 450046,China;College of Biology and Food,Shangqiu Normal University,Shangqiu Henan 476000,China;Institute of Crop Molecular Breeding,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第9期74-84,共11页
Journal of Southwest University(Natural Science Edition)
基金
国家重点研发计划项目(2017YFD0301101,2023YFD2301502)
河南省科技攻关项目(232102110018)
河南省重大科技专项(221100110300)。
关键词
小麦
基因TaARF14
克隆
表达分析
wheat(Triticum aestivum L.)
TaARF14
cloning
expression analysis
