摘要
本论文旨在研究荞麦蜂花粉多糖(BWPP)在模拟体外胃肠消化过程中的抗氧化活性。采用DEAE-52纤维素柱对粗多糖进行分级纯化,经体外模拟胃、肠消化研究其在体外消化过程中的抗氧化活性的变化规律。结果表明经DEAE-52纤维素柱层析洗脱纯化后得到总糖含量最高组分BWPP-1,其主要由Glc、Xyl、Ara等组成,且红外光谱显示BWPP-1具有明显的多糖特征吸收峰。BWPP和BWPP-1在进行体外模拟胃肠消化时,模拟胃液消化60 min时,BWPP和BWPP-1的ABTS^(+)·清除率均达到最大值,分别为86.39%±1.28%、90.38%±2.78%,与其他消化时间的存在显著差异(P<0.05);60 min时对DPPH·清除率达到最大值,分别为86.86%±0.11%、89.58%±1.67%,且二者差异不显著(P>0.05);模拟肠液消化时,BWPP和BWPP-1对ABTS^(+)·清除率在60 min时达到最大值为88.67%±0.40%和91.82%±2.77%,且二者差异不显著(P>0.05);对DPPH·清除率在240 min时达到最大值57.11%±0.06%和65.67%±3.67%,二者差异显著(P<0.05);而在消化过程中,对铁离子还原能力均较弱。由此可得,BWPP和BWPP-1具有较好的ABTS^(+)·和DPPH·清除能力,且胃液消化时DPPH·清除能力略高于肠液消化,而肠液消化时的ABTS^(+)·清除能力略高于胃液消化。本研究为以荞麦蜂花粉为基料的功能性产品的开发提供理论依据。
This paper aimed to investigate the antioxidant activity of buckwheat bee pollen polysaccharides(BWPP)during the in vitro gastrointestinal digestion.The crude polysaccharides were purified using DEAE-52 cellulose column and their antioxidant activity during in vitro simulated gastric and intestinal digestion was studied.The results showed that after purification by DEAE-52 cellulose column chromatography,the highest total sugar content component BWPP-1 was obtained,which was mainly composed of Glc,Xyl,Ara,etc.The infrared spectrum showed that BWPP-1 had obvious polysaccharide characteristic absorption peaks.During the simulated in vitro gastrointestinal digestion,the ABTS^(+)·scavenging rates of BWPP and BWPP-1 reached their maximum values at 60 minutes of simulated gastric juice digestion,which were 86.39%±1.28%and 90.38%±2.78%,respectively.There was a significant difference compared to other digestion times(P<0.05).The DPPH·clearance rates reached their maximum values at 60 minutes,which were 86.86%±0.11%and 89.58%±1.67%,respectively,and the difference between the two was not significant(P>0.05).When simulating intestinal digestion,the maximum clearance rates of ABTS^(+)·by BWPP and BWPP-1 reached 88.67%±0.40%and 91.82%±2.77%at 60 minutes,and the difference between the two was not significant(P>0.05).The DPPH·scavenging rates reached their peak values at 240 minutes of 57.11%±0.06%and 65.67%±3.67%,with significant difference(P<0.05).During the digestion process,the ability to reduce iron ions was relatively weak.Based on these findings,it can be concluded that BWPP and BWPP-1 have good ABTS^(+)·and DPPH·scavenging abilities,and the DPPH·scavenging ability during gastric digestion is slightly higher than that during intestinal digestion,while the ABTS^(+)·scavenging ability during intestinal digestion is slightly higher than that during gastric digestion.This study provides a theoretical basis for the development of functional products based on buckwheat bee pollen.
作者
王华
孟晶晶
马云啸
张志威
于丽丽
张继星
周文喜
WANG Hua;MENG Jingjing;MA Yunxiao;ZHANG Zhiwei;YU Lili;ZHANG Jixing;ZHOU Wenxi(College of Life Science and Food Engineering,Inner Mongolia Minzu University,Tongliao 028000,China;Buckwheat Industry Engineering Research Center of Inner Mongolia Minzu University,Tongliao 028000,China;Tongliao Institute of Agriculture and Animal Husbandry Science,Tongliao 028015,China)
出处
《食品工业科技》
CAS
北大核心
2024年第19期58-65,共8页
Science and Technology of Food Industry
基金
内蒙古自然科学基金研究项目(2024MS03060,2019MS03079)
内蒙古自治区高等学校教学研究项目(NJZY19149)
内蒙古自治区直属高校基本科研业务费项目(GXKY22152,23Z026)。
关键词
荞麦蜂花粉
多糖
纯化
体外模拟消化
抗氧化活性
buckwheat bee pollen
polysaccharide
purification
in vitro simulated digestion
antioxidant activity
