摘要
[目的]研究红托竹荪液体培养基中碳源、氮源及培养条件对其生长的影响,以建立红托竹荪高效液体培养体系。[方法]以红托竹荪固体母种菌丝为对象,采用液体培养基振荡培养的方法,通过测定液体培养后菌丝生长量及其在新培养基的恢复生长率等指标,研究预培养时间、不同碳源与氮源对液体培养效果的影响。[结果]液体培养中,接种块预培养6~8 d有助于菌丝球的形成与生长;菌丝生长最适碳源为浓度2.5%海藻糖,菌丝生长量与液体培养菌丝在栽培种培养基上的恢复生长能力均高于所用其他糖类处理;菌丝生长最适氮源为蛋白胨与巨菌草粉,蛋白胨2.0 g/L+巨菌草粉70.0 g/L的组合菌丝生长量、液体培养菌丝在栽培种培养基上的恢复生长能力分别达到0.7 g/L、100%。[结论]对液体菌种在红托竹荪及其他食用菌上的应用具有借鉴作用。
[Objective]The aim was to study the effects of carbon source,nitrogen source and culture conditions on the growth of Dictyophora rubra in liquid medium,in order to establish an efficient liquid culture system for D.rubrovalvata.[Method]Using the solid mycelium of D.rubrovalvata as the object,the effect of liquid culture was investigated by measuring the mycelial growth rate in liquid medium with different carbon and nitrogen sources and mycelium pellet recovery growth rate in the new culture medium.[Result]The results showed that in liquid culture,pre cultivation of inoculum blocks for 6-8 days facilitates the mycelial formation and growth.The most suitable carbon source for mycelial growth was trehalose,when its application concentration was 2.5%,the mycelial growth and the recovery growth ability were higher than those of other sugar treatments used.The most suitable nitrogen sources for mycelial growth was peptone and Cenchrus fungigraminus clippings.The mycelial growth rate of the recovery growth ability of liquid culture mycelium on the cultivation medium reached 0.7 g/L and 100%,respectively,in liqiud medium with protein peptone 2.0 g/L+giant fungus grass powder 70.0 g/L.[Conclusion]The research provided references value for the application of liquid bacterial strains in D.rubrovalvata and other edible mushrooms.
作者
唐璇
潘莉
刘晓星
陈广财
邓莉杰
韩美丽
霍秀娟
TANG Xuan(Guangxi Gaofeng State Owned Forest Farm,Nanning,Guangxi530007)
出处
《园艺与种苗》
CAS
2024年第8期20-23,共4页
Horticulture & Seed
基金
广西科技计划项目(桂科AB21076019)
广西农科院稳定资助科研团队项目(桂科农2021YT095)。
关键词
红托竹荪
液体培养
海藻糖
巨菌草
预培养
Dictyophora rubrovalvata
Liquid culture
Trehalose
Cenchrus fungigraminus
Preculture
