摘要
目的建立绵马贯众药材超高效液相色谱(UPLC)特征图谱及含量测定方法,对不同产地绵马贯众药材进行综合评价。方法采用UPLC对15批不同产地绵马贯众药材进行特征图谱测定,并结合聚类分析、偏最小二乘法分析对其质量进行评价,同时采用紫外分光光度法进行总黄酮含量测定。结果所建立的特征图谱共确定了21个共有峰,并通过对照品比对指认了其中4个成分。聚类分析及偏最小二乘法分析显示,辽宁省鞍山市、吉林省延边朝鲜族自治州、黑龙江省佳木斯市、吉林省吉林市样品具有一定的一致性,而总黄酮含量测定结果显示,不同产地之间样品含量差异较大。结论建立绵马贯众药材的UPLC特征图谱及总黄酮含量测定方法,可用于全面评价不同产地绵马贯众药材的质量。不同产地绵马贯众样品质量较为接近,但3个产地药材总黄酮含量差异较大。
Objective To establish UPLC specific chromatogram method and content determination method of Dryopteridis Crassirhizomatis Rhizoma;To comprehensively evaluate the Dryopteridis Crassirhizomatis Rhizoma from different producing areas.Methods UPLC method was used to establish the specific chromatogram of 15 batches of Dryopteridis Crassirhizomatis Rhizoma from different producing areas.The quality was evaluated by cluster analysis and partial least square analysis.The content of total flavonoids was determined by ultraviolet spectrophotometry.Results The established specific chromatogram identified a total of 21 common peaks,and identified 4 components through comparison with control samples.The clustering and partial least squares analysis showed that the samples from Anshan City in Liaoning Province,Yanbian Korean Autonomous Prefecture in Jilin Province,Jiamusi City in Heilongjiang Province,Jilin City in Jilin Province had a certain consistency.The results of total flavone content determination showed that the content of samples from different producing areas was different.Conclusion The established UPLC characteristic chromatogram and content determination method of total flavone content of Dryopteridis Crassirhizomatis Rhizoma can reflect the quality of Dryopteridis Crassirhizomatis Rhizoma in different places.The quality of samples from different origins is similar,but the contents of the three producing areas fluctuated greatly.
作者
曹斯琼
邱韵静
何嘉莹
钟春琳
徐敏
Cao Siqiong;Qiu Yunjing;He Jiaying;Zhong Chunlin;Xu Min(Guangdong Yifang Pharmaceutical Co.,LTD.,Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528000,China)
出处
《国际中医中药杂志》
2024年第9期1187-1192,共6页
International Journal of Traditional Chinese Medicine
基金
工信部2022年产业技术基础公共服务平台项目-中药全产业链质量技术服务平台(2022-230-221)
2022年佛山市南海区重点领域科技攻关专项(2022年)。
关键词
绵马贯众
特征图谱
含量测定(中药)
质量控制
Dryopteridis Crassirhizomatis Rhizoma
Specific chromatogram
Assay(TCD)
Quality control
