摘要
背景与目的:近年来,肝内胆管癌(ICC)发病率不断增加,因发病隐匿、早期症状不典型,ICC患者就诊时多属中晚期,预后较差。ICC细胞系是一种有价值的体外模型系统,广泛应用于ICC的基础与临床研发。通过对相关细胞系进行深入的研究及药物筛选,可望改善ICC患者预后。越来越多的证据表明肿瘤细胞的形态结构对于肿瘤的发生、发展及对药物的敏感性方面有较大影响。目前国内建立的中国人源ICC细胞系数量不多,对这些细胞系的特性鉴定不充分,特别在细胞的超微结构方面。因此,本研究探讨ICC细胞系ICC-X1的形态及超微结构特征,以期为后续研究提供参考。方法:对建立的新型中国人源ICC细胞系ICC-X1进行培养及处理。分别用光学显微镜、扫描电镜和透射电镜对ICC-X1细胞的显微结构、细胞的表面形态和超微结构以及细胞膜、细胞质和细胞器等胞内超微结构进行观察及分析。结果:光学显微镜下,ICC-X1呈贴壁生长,细胞形态以多角形及短梭形为主,其中单核细胞多见,细胞核大,细胞质少,核质比增加;营养充足的情况下细胞可出现叠层生长,失去接触抑制的特性。扫描电镜下,ICC-X1细胞周围可见丰富的微绒毛,微绒毛的粗细、长短比较均匀,且细胞有较长的伪足。透射电镜下,ICC-X1细胞内核大,核偏移,染色质电子密度不均,可见丰富线粒体及核糖体。结论:本研究获得了ICC-X1细胞形态学及超微结构的详细资料,为探讨ICC细胞结构与功能的关系以及ICC耐药机制的研究提供了线索与思路。
Background and Aims:In recent years,the incidence of intrahepatic cholangiocarcinoma(ICC)has increased.Due to its insidious onset and atypical early symptoms,most ICC patients are diagnosed at a late stage,leading to a poor prognosis.ICC cell lines are valuable in vitro models widely used in basic and clinical research on ICC.In-depth studies and drug screening of these cell lines may improve patient outcomes.Increasing evidence suggests that the morphological structure of tumor cells significantly impacts tumor development,progression,and drug sensitivity.Only a limited number of ICC cell lines derived from Chinese patients have been established,and their characteristics,particularly their ultrastructural features,are not fully characterized.Therefore,this study investigated the morphological and ultrastructural characteristics of the ICC-X1 cell line,aiming to provide a reference for future research.Methods:The newly established Chinese human ICC cell line ICC-X1 was cultured and processed.The microscopic structure,surface morphology,and ultrastructure of ICC-X1 cells,including the cell membrane,cytoplasm,and organelles,were observed and analyzed using light,scanning,and transmission electron microscopy,respectively.Results:Under optical microscopy,ICC-X1 cells exhibited adherent growth,predominantly polygonal and short spindle-shaped morphology,with a high prevalence of mononuclear cells,large nuclei,and scant cytoplasm,resulting in an increased nucleocytoplasmic ratio.When adequately nourished,the cells showed layered growth and lost contact inhibition.Scanning electron microscopy revealed an abundance of microvilli surrounding the ICC-X1 cells,with uniform thickness and length and long pseudopodia.Transmission electron microscopy showed that ICC-X1 cells had giant,eccentrically placed nuclei with uneven electron density of chromatin,and abundant mitochondria and ribosomes were observed.Conclusion:This study provides detailed data on the morphology and ultrastructure of the ICC-X1 cell line,offering insights and perspectives for exploring the relationship between ICC cell structure and function and investigating the mechanisms of ICC drug resistance.
作者
赵振杰
张波
张泽亮
柴长鹏
骆伟
苗龙
王正峰
徐浩
ZHAO Zhenjie;ZHANG Bo;ZHANG Zeliang;CHAI Changpeng;LUO Wei;MIAO Long;WANG Zhengfeng;XU Hao(General Surgery Ward 4,First Hospital of Lanzhou University,Lanzhou 730000,China;Department of Hepatobiliary and Pancreatic Surgery,Zhejiang Provincial Hospital of Traditional Chinese Medicine,Hangzhou 310006,China)
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2024年第8期1258-1263,共6页
China Journal of General Surgery
基金
甘肃省自然科学基金资助项目(23JRRA0929,23JRRA1601)
兰州大学第一医院院内基金资助项目(ldyyyn2022-12,ldyyyn2021-61)。
