摘要
在奶牛养殖过程中,物理性致伤、细菌感染等因素作用于机体组织或器官,使皮肤或黏膜组织等完整性被破坏而导致组织损伤和创伤的出现。损伤愈合是一个复杂的过程,需要多种细胞的相互协作以及多种细胞因子的参与。其中,巨噬细胞和肌成纤维细胞的相互作用对于组织修复是不可或缺的。天然免疫系统中的模式识别受体NOD样受体蛋白3(Nod-like receptor protein 3,NLRP3)可能对机体组织修复过程具有调控作用。本试验通过体外培养奶牛肌成纤维细胞及M1型骨髓源巨噬细胞,收集细胞培养上清液进行共培养,检测M1型骨髓源巨噬细胞中细胞因子的分泌、肌成纤维细胞生长因子蛋白及mRNA表达。通过使用NLRP3抑制剂MCC950探讨NLRP3在2种细胞相互作用过程中发挥的调控机制。结果显示,本试验成功建立了体外培养奶牛肌成纤维细胞方法。肌成纤维细胞培养上清液(myofibroblast conditioned medium,MFbCM)能够调节M1型巨噬细胞促炎因子分泌活动,M1型巨噬细胞培养上清液(M1macrophage conditioned medium,M1oCM)能够调节肌成纤维细胞生长因子表达,且NLRP3参与2种细胞间相互作用。结果表明,NLRP3在肌成纤维细胞和M1型骨髓源巨噬细胞的相互作用过程中具有一定的调控作用。
During the process of dairy farming,various factors such as physical injury and bacterial infection act upon body tissues or organs,leading to the disruption of skin or mucous tissue integrity and subsequent tissue injury and trauma.The healing of these injuries is a complex process that necessitates the coordinated efforts of different cells and involvement of diverse cytokines.Among them,the interaction between macrophages and myofibroblasts is indispensable for efficient tissue repair.Nod-like receptor protein 3(NLRP3),apattern recognition receptor in the innate immune system,may play a regulatory role in modulating this intricate process.In this study,cow myofibroblasts and M1type bone marrow-derived macrophages were cultured in vitro,followed by collection of cell culture supernatant for co-culture analysis.Both cytokine secretion levels in M1 type bone marrow-derived macrophages as well as expression patterns levels of myofibroblast growth factor protein and mRNA were detected.The regulatory mechanism underlying NLRP3involvement in mediating interactions between these two cell types was investigated using NLRP3 inhibitor MCC950.The results showed that an effective method for culturing cow muscle fibroblasts in vitro was successfully established and myofibroblast conditioned medium(MFbCM)could regulate M1macrophage secretion profiles.Moreover,M1macrophage conditioned medium(M1oCM)was found to influence myofibroblast growth factor expression levels.Our findings suggest that NLRP3plays a significant regulatory role during crosstalk between myofibroblasts and M1-type pro-inflammatory macrophages.
作者
白云洁
赵佳敏
巩志国
包文慧
于琢雅
王超
毛伟
张双翼
刘博
BAI Yunjie;ZHAO Jiamin;GONG Zhiguo;BAO Wenhui;YU Zhuoya;WANG Chao;MAO Wei;ZHANG Shuangyi;LIU Bo(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010010,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2024年第7期1507-1513,1520,共8页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(32160854)
内蒙古自治区直属高校基本科研业务费资助项目(BR231510)
中央引导地方科技发展资金资助项目(2022ZY0200)。
