摘要
阴沟肠杆菌(Enterobacter cloacae)是一种机会致病菌,有导致鲻鱼等水产动物大量死亡等严重问题。从福建某水产养殖场污水中分离到一株新型裂解性阴沟肠杆菌噬菌体ZX14并对其进行生物学特性及基因组分析,探索ZX14在防治多重耐药阴沟肠杆菌感染的应用价值。噬菌体ZX14能高效裂解阴沟肠杆菌,效价高达1.5×10^(10)PFU/mL,潜伏期为20 min,爆发量为45 PFU/cell,且在50℃、pH 5~12等环境下具有良好稳定性和适应性。基因组分析显示,ZX14基因组全长173 679 bp, GC含量为39.92%,共编码294个开放阅读框(open reading frame, ORF)和10个tRNA,不含耐药基因和毒力基因,其中122个ORF被注释为功能蛋白。此外,该噬菌体可跨种裂解阴沟肠杆菌、路德维希肠杆菌(Enterobacter ludwigii)和神户肠杆菌(Enterobacter kobei),并对这三种细菌在体外都显示出高效抗菌活性。研究首次报道了一株可跨种裂解细菌的噬菌体ZX14,该噬菌体具有广阔应用前景,有望为防治多重耐药阴沟肠杆菌感染提供新的解决方案。
Enterobacter cloacae is an opportunistic pathogen that can cause serious problems such as sepsis and urinary tract infections in newborns.In this study,we isolated and purified lytic Enterobacter cloacae phage from a fish market in Fujian Province,China.A new lytic Enterobacter cloacae phage ZX14 was isolated and identified.Its biological characteristics,genome analysis,and its application value in the prevention and treatment of multidrug-resistant Enterobacter cloacae infections was explored.The identification results showed that the phage could efficiently lyse Enterobacter cloacae and the titer was as high as 1.5×10^(10) PFU/mL.Analysis of its biological characteristics showed that the incubation period of ZX14 was 20 min and the burst size was 45 PFU/cell when ZX14 was infected with 2025 Enterobacter cloacae strain.It has good stability and adaptability at 50℃,pH 5-12 and other environmental conditions.Genomic analysis showed that the full length of the ZX14 genome was 173679 bp with 39.92%guanine-cytosine(GC)content.A total of 294 open reading frames(ORFs)and 10 tRNAs were predicted,of which 122 ORFs were structurally similar to known functional proteins.No drug resistance gene and no virulence gene were detected,and the safety was good.In addition,it was found that the phage could also lyse Enterobacter ludwigii and Enterobacter kobei by lytic spectroscopy.In conclusion,this new lytic Enterobacter cloacae phage has a wide range of potential applications and is expected to provide a new solution for the prevention and treatment of Gram Enterobacter cloacae infections.
作者
郭开鑫
张金城
倪畅
沈茫茫
周昕
GUO Kaixin;ZHANG Jincheng;NI Chang;SHEN Mangmang;ZHOU Xin(Veterinary College,Yangzhou University,Yangzhou 225009,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2024年第8期14-26,共13页
China Biotechnology
基金
国家自然科学基金(32271435)
江苏省高等学校重点学科发展项目(PAPD)资助项目。
关键词
阴沟肠杆菌
裂解性噬菌体
生物学特性
基因组分析
Enterobacter cloacae
Lytic phage
Biological characteristics
Genomic analysis
