摘要
目的采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap-HRMS)技术分别对以水和50%甲醇溶液为溶剂提取的葛根-丹参药对样品进行化学成分分析。方法采用Thermo Scientific Hypersil GOLD C_(18)色谱柱(150 mm×2.1 mm,1.9μm),以0.1%甲酸水-0.1%甲酸乙腈溶液为流动相进行梯度洗脱,流速为0.3 mL·min^(-1),柱温为30℃。采用加热电喷雾离子源(H-ESI),正负离子检测模式。结果从水提和50%甲醇醇提样品溶液中均鉴定出104个化合物,其中103个化合物为共有成分,各有1个差异性成分,分别为水提样品中的刺槐苷与50%甲醇醇提样品中的降丹参酮(或其同分异构体)。共鉴定出的105个化合物,包括黄酮类成分36个、苯丙素类成分20个、萜类成分24个和其他类成分25个。结论本研究首次采用UPLC-Q-Orbitrap-HRMS技术对葛根-丹参药对的主要成分进行鉴定,可为葛根-丹参药对的药效成分研究提供科学依据。
Objective To analyze the chemical components of Gegen-Danshen herb pair extracted with water and 50%methanol solution by UPLC-Q-Orbitrap-HRMS.Methods Thermo Scientific Hypersil GOLD C_(18) column(150 mm×2.1 mm,1.9μm)was used for gradient elution with 0.1%formic acid water-0.1%formic acid acetonitrile solution as the mobile phase.The flow rate was 0.3-1 mL·min and the column temperature was 30℃.A heated electrospray ionization(H-ESI)source was used for HRMS analysis in both positive and negative ion modes.Results In both water and 50%methanol extract samples,104 compounds were tentatively identified:103 common components,and one differentials component,namely acacetin in the water extract and nortanshinone(or its isomers)in 50%methanol extract.Among the identified 105 compounds,there were 36 flavonoids,20 phenylpropanoids,24 terpenoids and 25 other components.Conclusion The main components of Gegen-Danshen herb pair are characterized by UPLC-Q-Orbitrap-HRMS for the first time,which provides reference for the discovery of bioactive components in Gegen-Danshen herb pair.
作者
卢洋
邓小惠
吴梦瑶
陈萱
陈祥雯
杨宇辉
曹纪亮
万丽
LU Yang;DENG Xiao-hui;WU Meng-yao;CHEN Xuan;CHEN Xiang-wen;YANG Yu-hui;CAO Ji-liang;WAN Li(School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine,Chengdu 611137;School of Pharmacy,Shenzhen Technology University,Shenzhen Guangdong 518118)
出处
《中南药学》
CAS
2024年第9期2380-2386,共7页
Central South Pharmacy
基金
国家自然科学基金青年科学基金项目(No.82104362)
深圳技术大学孔雀人才启动项目(No.GDRC202119)
深圳市科技计划面上项目(No.JCYJ20220530153201003)
博士后留深科研资助项目(No.20211063010055)。
