摘要
采用常规组织分离法分离山豆根(Sophora tonkinensis Radix)菌核病病原菌,利用柯赫式法则进行致病性测定,基于形态特征、菌丝融合群测定、细胞核荧光染色以及rDNA-ITS序列分析进行病原菌的鉴定,并利用菌丝生长速率法测定5种杀菌剂对病原菌的抑制活性。结果表明,山豆根菌核病的病原菌为立枯丝核菌(Rhizoctonia solani),属于融合群AG-1。98%恶霉灵可溶性粉剂(SP)、75%肟菌·戊唑醇水分散粒剂(WG)、250 g/L吡唑醚菌酯乳油(EC)、10亿/g哈茨木霉菌悬浮剂(SC)、50%多菌灵可湿性粉剂(WP)对病原菌菌丝的生长均有较好的抑制效果,抑制中浓度(EC_(50))分别为0.0611、0.5757、0.0748、0.2843、0.7405 mg/L。
The pathogens from Sophora tonkinensis Radix were isolated by conventional tissue separation method,the pathogenicity was determined by Koch’s formula,and the pathogens were identified based on morphological characteristics,mycelium fusion group determination,nuclear fluorescence staining and rDNA-ITS sequence analysis.The inhibitory activities of 5 fungicides against patho-gens were measured by the mycelium growth rate method.The results showed that the pathogen was Rhizoctonia solani,belonging to the fusion group AG-1.98%hymexazol soluble power(SP),75%oxime·pentazolol water dispersible granule(WG),250 g/L pyra-clostrobin emulsifiable concentrate(EC),1 billion/g Trichoderma harziana suspension concentrate(SC),and 50%carbendazim wet-table power(WP)all had good inhibitory effects on the growth of pathogenic fungi mycelia.The inhibitory medium effective concentra-tions(EC_(50))were 0.0611,0.5757,0.0748,0.2843,0.7405 mg/L,respectively.
作者
宋利沙
蒋妮
张占江
韦树根
丘卓秋
黄琦
詹鑫婕
潘丽梅
SONG Li-sha;JIANG Ni;ZHANG Zhan-jiang;WEI Shu-gen;QIU Zhuo-qiu;HUANG Qi;ZHAN Xin-jie;PAN Li-mei(Guangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-Di Herbs/Guangxi Traditional Chinese Medicine Breeding Technology Innovation Center/National Center for Traditional Chinese Medicine Inheritance and Innovation,Nanning 530023,China)
出处
《湖北农业科学》
2024年第9期89-94,101,共7页
Hubei Agricultural Sciences
基金
广西中医药适宜技术开发与推广项目(GZSY21-03)
广西卫生健康委员会重点实验室建设项目(ZJC2020003)
中医药多学科交叉创新团队项目(GZKJ2305)。
