摘要
【背景】植物精油是一种具有挥发性气味的次生代谢产物,具有环保、天然及无残留等特性,广泛应用于食品、农业及医药等行业,在人们生活中发挥着重要作用。【目的】研究紫苏[Perilla frutescens(L.)Britt]和毛地黄鼠尾草(Salvia digitaloides Diels)植物精油挥发性成分、抗真菌和细胞毒作用。【方法】采用水蒸汽蒸馏法提取精油,用气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)联用仪检测挥发性成分;通过菌丝生长速率法评定抗真菌作用;使用噻唑蓝(methyl thiazolyl tetrazolium,MTT)比色法评估精油的细胞毒活性。【结果】紫苏精油中主要成分为紫苏酮(67.84%)和异白烯苏酮(14.95%),毛地黄鼠尾草精油中主要成分为石竹素(9.59%)、α-荜澄茄醇(8.84%)和棕榈酸(7.24%)。研究发现植物精油都呈现不同程度的抗菌作用,其中紫苏精油活性最强,对细极链格孢菌(Alternaria tenuissima)、油菜菌核菌(Sclerotinia sclerotiorum)和人参锈腐菌(Cylindrocarpon destructans)表现出较高的抑制效果,其有效中浓度(median effective concentrations,EC_(50))分别为32.78、43.34和45.19μg/mL,毛地黄鼠尾草精油对腐皮镰刀菌(Fusarium solani)的EC_(50)为97.68μg/mL;毛地黄鼠尾草精油和紫苏精油对K562细胞的抑制中浓度(median inhibitory concentration,IC_(50))分别为282.69μg/mL和352.79μg/mL。【结论】植物精油具有良好的生物学特性。
[Background]Plant essential oils are secondary metabolites with a volatile odor,which are environmentally friendly,natural,and residue-free,and it is widely used in food,gariculture and medicene,play an important role in people’s live.[Objective]To study the volatile components in the essential oils from Perilla frutescens(L.)Britt and Salvia digitaloides Diels and examine the anti-fungal and cytotoxic effects of the essential oils.[Methods]Essential oils were extracted by the steam distillation method,and the chemical components in the essential oils were analyzed by gas chromatography-mass spectrometry(GC-MS).The anti-fungal and cytotoxic effects of the essential oils were evaluated by the mycelial growth rate and the methyl thiazolyl tetrazolium(MTT)colorimetric assay.[Results]The main chemical components in the essential oil of P.frutescens were perillaketone(67.84%)and isoegomaketone(14.95%),while those of S.digitaloides Diels were caryophyllene oxide(9.59%),α-cadinol(8.84%),and palmitic acid(7.24%).The essential oils had varying degrees of antimicrobial effects.Specifically,the essential oil of P.frutescens had the strongest activity,with the median effective concentration(EC_(50))of 32.78,43.34 and 45.19μg/mL against Alternaria tenuissima,Sclerotinia sclerotiorum,and Cylindrocarpon destructans,respectively.The EC_(50) of the essential oil of S.digitaloides Diels against Fusarium solani was 97.68μg/mL.The median inhibitory concentration(IC_(50))of the essential oils of S.digitaloides Diels and P.frutescens against K562 cells were 282.69μg/mL and 352.79μg/mL,respectively.[Conclusion]The essential oils of S.digitaloides Diels and P.frutescens have strong biological activities.
作者
王光慧
余帅
王梓懿
周红静
韩科展
李䶮
WANG Guanghui;YU Shuai;WANG Ziyi;ZHOU Hongjing;HAN Kezhan;LI Yan(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,Guizhou,China;Natural Products Research Center of Guizhou Province,Guizhou Provincial Engineering Research Center for Natural Drugs,Guiyang 550014,Guizhou,China;College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2024年第9期3409-3421,共13页
Microbiology China
基金
延安市科技计划(2023-NYGG-008)
贵州省科技计划(黔科合支撑[2020]1Y173)。
关键词
紫苏精油
毛地黄鼠尾草精油
抗真菌
细胞毒活性
essential oil of Perilla frutescens(L.)Britt
essential oil of Salvia digitaloides Diels
anti-fungal
cytotoxic activity
