摘要
促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)级联是植物中重要的信号传递通路,参与调控植株的多种生物和非生物胁迫。为研究SlMAPK3对番茄耐旱方面的影响,本试验以野生型番茄M82及其过表达SlMAPK3株系OE1、OE3、OE4为研究对象,定植田间后,6~7片真叶期进行中度干旱处理保持田间持水量45%~55%,于开花期,统计植株的高度、结果量,比较了不同株系之间果实大小、果实产量以及植株叶片抗氧化活性差异。在田间条件研究中发现,SlMAPK3过表达番茄株系的耐性更强,暗示SlMAPK3在番茄抗旱中发挥重要调控作用,为番茄抗逆育种奠定基础。
The mitogen-activated protein kinase(MAPK)cascade is an important signal transmission pathway in plants.MAPK cascade participates in the regulation of a variety of biotic and abiotic stresses in plants.In order to study the influence of SlMAPK3 on drought tolerance in tomatoes planted in field,wild-type tomato M82 and its overexpression SlMAPK3 lines OE1,OE3,OE4 were used in this research.After planting in the field,6~7 true leaves were treated with moderate drought to maintain the water holding capacity of the field at 45%~55%.At flowering stage,plant height and fruit quantity were counted,and the differences of fruit size,fruit yield and antioxidant activity were compared from leaves in different lines.In field research,it was found that tomato lines of overexpressing SlMAPK3 were more tolerant to drought stress,suggesting that SlMAPK3 plays an important regulatory role in tomato drought resistance,laying the foundation for tomato stress-resistant breeding.
作者
张龙
吴丹
郑寅
李云洲
梁燕
Zhang Long;Wu Dan;Zheng Yin;Li Yunzhou;Liang Yan(College of Agriculture,Guizhou University,Guiyang,550025;College of Horticulture,Northwest A&F University,Yangling,712100;College of Animal Science,Guizhou University,Guiyang,550025)
出处
《分子植物育种》
CAS
北大核心
2024年第19期6368-6374,共7页
Molecular Plant Breeding
基金
国家自然科学基金项目(32060679)
贵州大学培育项目(贵大培育[2019]52号)
贵州大学培育项目(黔科合平台人才[2017]5788-28)
贵州大学人才引进科研项目(贵大人基合字[2017]50号)
大学生创新创业项目(S202010657039)共同资助。
关键词
番茄
MAPK3
转基因
抗旱性
Tomato
MAPK3
Genetically modified
Drought resistance
