摘要
为评估紫花风铃木的抗旱能力,本研究以其为材料进行控水试验,试验开始后每隔3 d测定栽培基质相对含水量,叶绿素相对含量(SPAD值),丙二醛(MDA)含量,可溶性糖和脯氨酸(Pro)含量以及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,探究其对干旱胁迫的生理响应。结果表明,在干旱初期,MDA含量上升,植物细胞膜受到一定程度的损伤,可溶性糖和Pro等渗透调节物质含量增加,SOD和CAT活性增加,表现出一定的抗旱耐受性;在栽培基质相对含水量低于33.71%后,可溶性糖和Pro含量继续增加,但SOD和CAT活性逐渐下降,MDA维持在较高水平,表明紫花风铃木干旱耐受达到极限。综合以上结果表明,干旱胁迫初期,紫花风铃木可以通过渗透调节和激活抗氧化酶活性的方式,对干旱胁迫表现出一定耐受性;随着胁迫程度的增加,其干旱耐受达到极限,SOD和CAT活性下降,其生长受到抑制。
To evaluate the drought resistance of Handroanthus impetiginosus.The water control test was carried out with Handroanthus impetiginosus as the material.After the test,the relative water content,relative chlorophyll content(SPAD),malondialdehyde(MDA)content,soluble sugar and proline(Pro)content and the activities of superoxide dismutase(SOD)and catalase(CAT)of the cultivated substrate were measured every 3 days to explore the physiological response of Handroanthus impetiginosus to drought stress.The results showed that,in the early stages of drought,the content of MDA increased,plant cell membranes were damaged,soluble sugars and Pro and other osmotic regulatory substances increased,and the activities of SOD and CAT increased,demonstrating a certain degree of drought resistance.After the relative moisture content of the cultivation substrate was below 33.71%,soluble sugars and Pro increased,but the activities of SOD and CAT gradually decreased from their peak,and MDA remained at a high level,indicating that Handroanthus impetiginosus may have reached its drought tolerance limit.These results indicated that Handroanthus impetiginosus could be tolerant to drought stress by osmotic regulation and activation of antioxidant enzyme activity at the early stage of drought stress.With the increase of stress degree,the drought tolerance reached the limit,and the activities of antioxidant enzymes SOD and CAT decreased,which inhibited the growth of Handroanthus impetiginosus.
作者
窦锁柱
余兴生
DOU Suozhu;YU Xingsheng(Shenzhen Guoyi Park Construction Co.,Ltd.,Shenzhen 518040,China;Shenzhen Municipal Group Co.,Ltd.,Shenzhen 518000,China)
出处
《安徽农学通报》
2024年第19期57-60,共4页
Anhui Agricultural Science Bulletin
基金
深圳市南山区城管局科研项目“深圳大沙河生态廊道景观示范”(2022)。
关键词
紫花风铃木
干旱胁迫
渗透调节
抗氧化酶
Handroanthus impetiginosus
drought stress
osmotic regulation
antioxidant enzymes
