摘要
利用液体培养基对芒果花粉进行离体培养的方法,探究温度和芒果花期防控白粉病、霜霉病、炭疽病、蓟马常用的药剂对台农1号芒果花粉萌发及花粉管生长的影响,筛选低毒性的药剂。结果表明,温度低于15.0℃时台农1号花粉不萌发,15.0~20.0℃花粉萌发率缓慢增加,花粉萌发率及花粉管长度在27.5℃时达到峰值,温度高于27.5℃之后花粉萌发率开始下降,但在40.0℃时仍有35.74%的萌发率。31种芒果花期常用杀菌、杀虫剂单独添加到培养基中发现,与不添加药剂的空白对照相比,英腾花粉萌发率及花粉管长度均无显著影响;势克、安泰生、美甜花粉萌发率均无显著影响,但花粉管长度均显著降低;有9种药剂花粉萌发率及花粉管长度均显著降低;18种药剂花粉完全不萌发。药剂复配添加到培养基中发现,安拓+爱苗+英腾、安泰生+爱苗+英腾2个药剂组合花粉萌发率均无显著降低,但花粉管长度均显著降低;11个药剂组合花粉萌发率及花粉管长度均显著降低;3个药剂组合花粉完全不萌发。
The aim of this study was to explore the effects of temperature and common pesticides used to control powdery mildew,downy mildew,anthracnose and thrips on pollen germination and pollen tube growth of‘Tainong 1'using liquid culture medium in vitro,to screen low-toxicity agents.The results showed that pollen did not germ when the temperature was lower than 15.0℃,pollen germination rate increased slowly with the increase of temperature between 15.0℃and 20.0℃,and the pollen germination rate and pollen tube length reached the peak at 27.5℃.When the temperature was higher than 27.5℃,the pollen germination rate and pollen tube length both decreased with the increase of temperature.The germination rate was 35.74%at 40.0℃.In the study of 31 commonly used pesticides added to the culture medium respectively,it was found that under the experimental concentration,the effects of different pesticides on pollen germination and pollen tube growth were significantly different.Compared with the blank control without adding fungicides,“yingteng”had no significant inhibition on pollen germination and pollen tube growth,“shike”“antaisheng”“meitian”had no significant effect on the germination rate,but there were significant inhibition on pollen tube growth,9 types of pesticides had significant inhibition on both pollen germination and pollen tube growth,18 types of pesticides made pollen not germinate at all.It was found that the pollen germination rate of 2 combinations was not significantly decreased,but the pollen tube length was significantly decreased,the pollen germination rate and the pollen tube length were significantly decreased in 11 combinations,and the pollen did not germinate at all in 3 combinations.
作者
蔡昭艳
董龙
农贵雄
王葫青
岑宝林
苏伟强
方位宽
黄章保
任惠
CAI Zhaoyan;DONG Long;NONG Guixiong;WANG Huqing;CEN Baolin;SU Weiqiang;FANG Weikuan;HUANG Zhangbao;REN Hui(Institute of Horticultural,Guangxi Academy of Agricultural Sciences,Nanning 530007;Agricultural Comprehensive Service Center of Naman Town People’s Government,Tianyang District,Baise City;Longlin Ethnic Autonomous County Fruit Tea Industry Production Technical Guidance Station;Agricultural Service Center of Youping Township People’s Government,Leye County)
出处
《中国果树》
2024年第9期57-63,70,共8页
China Fruits
基金
国家重点研发计划(2022YFD1100106)
财政部和农业农村部:国家现代农业产业技术体系(CARS-31)
广西科技重大专项(桂科AA22068098-3)
广西农业科学院基本科研业务专项(桂农科2021YT046)
广西农业科学院科技发展基金(桂农科2017JM16)
广西特色作物试验站“广西田东芒果试验站”。
关键词
芒果
花粉
温度
杀菌剂
杀虫剂
mango
pollen
temperature
fungicide
insecticide
