摘要
植物与微生物互作在缓解非生物胁迫和提高作物生产力等方面发挥重要作用,目前关于丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)诱导植物Cu吸收以及由此减少Cd吸收方面的研究鲜见报道.在添加和不添加Cd条件下分别设置对照(CK)、接种AMF(AM)、添加Cu(Cu)、AM+Cu等处理的盆栽实验,探究Cd胁迫下接种AMF或外源添加Cu对紫云英生长和Cd吸收转运的影响.结果显示,接种AMF及添加Cu元素均促进植物根系的发育,植株的生物量显著增加,并在不同程度上提高了紫云英植株内可溶性糖和脯氨酸含量、过氧化物酶和超氧化物歧化酶的活性,降低了过氧化氢含量、地上部分Cd浓度以及减轻地下部分Cd向地上部分的转移.其中,两者同时存在的促进效应大于单独存在处理组.本研究表明Cu元素和AMF有利于紫云英在Cd胁迫下生长,通过降低其活性氧分子、提高渗透调节物质含量和抗氧化酶活性、降低金属Cd离子在地上部的累积,从而缓解金属污染对紫云英造成的损伤. Cu元素提高了AMF与植物的共生效应,可能与菌根介导增强Cu吸收和由此产生的金属耐性有关.本研究结果有助于更深入地了解丛枝菌根共生修复重金属污染的机理.(图4表1参49)
Plant-microbial interactions play a critical role in alleviating abiotic stress and enhancing crop productivity.However,there are limited reports on the induction of plant copper(Cu)uptake by arbuscular mycorrhizal fungi(AMF)and the subsequent reduction in cadmium(Cd)uptake.In this study,we conducted a pot experiment with four treatments:control(CK),AMF inoculation(AM),Cu addition(Cu),and AM+Cu,with and without Cd addition.The aim was to explore the effects of AMF inoculation and exogenous Cu addition on the growth,absorption,and transport of Cd in Astragalus sinicus L.under Cd stress.Results showed that AMF inoculation and Cu supplementation significantly promoted root development,plant biomass,soluble sugar and proline content,and activities of peroxidase and superoxide dismutase.The contents of hydrogen peroxide and Cd in aboveground parts and the transfer of Cd from roots to shoots were reduced in the AMFand Cu-treated plants.The combined treatment had a greater promoting effect than the separate treatments.This study concluded that Cu and AMF are beneficial for the growth of A.sinicus under Cd stress and mitigate metal pollution damage by reducing active oxygen molecules,increasing osmolytes content,and enhancing antioxidant enzyme activity,thereby reducing Cd ion accumulation in shoots.Cu enhances the symbiotic effect,potentially related to mycorrhiza-mediated enhanced Cu absorption and resulting metal tolerance.This study provides a further understanding of the mechanisms of heavy metal remediation by AMF.
作者
胡明慧
郭梦雅
李思瑶
蔡蕊
马坤坤
张溪溪
朱先灿
HU Minghui;GUO Mengya;LI Siyao;CAI Rui;MA Kunkun;ZHANG Xixi;ZHU Xiancan(College of Life Sciences,Anhui Normal University,Wuhu 241000,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2024年第4期795-801,共7页
Chinese Journal of Applied and Environmental Biology
基金
重要生物资源开发与利用研究安徽省重点实验室重点项目(swzy202001)资助。
关键词
丛枝菌根共生
重金属污染
植物与微生物修复
紫云英
arbuscular mycorrhizal fungi
heavy metal pollution
plant and microbial remediation
Astragalus sinicus L.
