摘要
目的:筛选新疆藁本多酚超声提取的最佳工艺,并对新疆藁本多酚的抗氧化能力进行评测。方法:以新疆藁本为原料,采用单因素法结合响应面优化法确定新疆藁本多酚最佳超声提取工艺,并用DPPH自由基清除率实验和Fe3+还原能力测试判断新疆藁本多酚抗氧化能力的强弱。结果:在25℃下,新疆藁本多酚超声提取的最优工艺为乙醇浓度62%,提取时间40 min,料液比1∶40(g·m L^(-1)),此时多酚提取率为3.43%。新疆藁本多酚对DPPH自由基的清除能力和Fe^(3+)的还原能力均随浓度增加呈上升趋势。新疆藁本多酚清除DPPH自由基的IC_(50)是0.37 mg·m L^(-1)。结论:本文建立的工艺验证试验结果与预测提取率接近,说明模型建立较好;新疆藁本多酚具备一定抗氧化能力,具有很好的开发应用前景。
Objective:Screened the optimal process for ultrasonic extraction of polyphenols from Conioselinum vaginatum,and evaluated the antioxidant activity.Methods:The optimal ultrasonic extraction process for C.vaginatum was determined by single factor method combined with response surface methodology(RSM).The antioxidant properties of C.vaginatum was evaluated by DPPH radical scavenging activity and Fe3+reduction test.Results:The best operating experiment conditions for C.vaginatum were as following:extraction time was 40 minutes,ethanol solutions was 62%,and the solid-liquid ratio was 1∶40(g·mL^(-1)).Under the above conditions,the extraction yields of polyphenols from C.vaginatum was 3.43%.The scavenging ability of C.vaginatum polyphenols on DPPH free radicals and the redution ability of Fe^(3+)exhibited an upward trend with the increase of concentration.The IC_(50)values of C.vaginatum for scavenging DPPH free radicals was 0.37 mg·mL^(-1).Conclusion:The established process validation test results were close to the predicted extraction rate,indicating that the model was well established.Polyphenols from C.vaginatum had certain antioxidant potential and had a good prospects of development and application.
作者
潘兰
贾月梅
周天源
兰卫
赵延龄
Pan Lan;Jia Yuemei;Zhou Tianyuan;Lan Wei;Zhao Yanling(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830017,China;The Center of Market Supervision and Evaluation of Xinjiang,Urumqi 830000,China)
出处
《中国野生植物资源》
CSCD
2024年第9期42-48,共7页
Chinese Wild Plant Resources
基金
新疆自然科学基金项目(2020D01B48)。
关键词
新疆藁本
多酚
响应面法
抗氧化
Conioselinum vaginatum
Polyphenols
RSM
Antioxidant
