摘要
背景肿瘤的准确诊断对患者治疗和预后至关重要。特殊染色和免疫组化染色各具优势,但两者单独使用时仍存在局限性。将两者相结合可能提供更全面、更准确的诊断信息,但其临床应用价值尚需系统评估。目的探讨特殊染色联合免疫组化染色在肿瘤诊断中的应用价值,评估其诊断效能。方法选取2021年1月至2023年12月在郑州颐和医院接受诊治的80例肿瘤患者,包括转移性肿瘤患者25例、原发性肝癌患者20例、血管瘤患者15例、内胆管瘤患者20例。对患者组织样本分别进行免疫组化染色、Masson染色、PAS染色和刚果红染色。以病理确诊结果为金标准,比较各染色方法的诊断效能,包括准确度、敏感度、特异度、阳性预测值和阴性预测值。采用ROC曲线分析各染色方法的诊断效能。结果特殊染色联合免疫组化染色的ROC曲线下面积最大(0.943),显示出最高的诊断效能。该方法的准确度为97.50%,敏感度为98.57%,特异度为90.00%,均在不同程度上高于单一染色方法(P<0.05)。单独使用免疫组化染色时的准确度为92.50%,敏感度为95.71%,特异度为70.00%。Masson染色、PAS染色和刚果红染色的准确度分别为82.50%、87.50%、80.00%,均低于特殊染色联合免疫组化染色(P<0.05)。结论特殊染色联合免疫组化染色在肿瘤诊断中具有较高的应用价值,能够显著提高诊断的准确度、敏感度和特异度,优于单一染色方法。该联合技术可为临床肿瘤诊断提供更可靠的依据。
Background Accurate diagnosis of tumors is crucial for patient treatment and prognosis.Special staining and immunohistochemistry staining each has its own advantages,but they still have limitations when used individually.Combining the two may provide more comprehensive and accurate diagnostic information;however,the clinical value of this approach requires systematic evaluation.Objective To explore the application value of special staining combined with immunohistochemistry staining in tumor diagnosis and to evaluate its diagnostic efficacy.Methods A total of 80 tumor patients treated at Zhengzhou Yihe Hospital from January 2021 to December 2023 were selected,including 25 cases of metastatic tumors,20 cases of primary liver cancer,15 cases of hemangiomas,and 20 cases of intrahepatic cholangiocarcinomas.Tissue samples from the patients were subjected to immunohistochemical staining,Masson staining,PAS staining,and Congo red staining.Using pathological diagnosis as the gold standard,the diagnostic efficacy of various staining methods was compared,including accuracy,sensitivity,specificity,positive predictive value,and negative predictive value.The diagnostic efficacy of each staining method was analyzed using ROC curves.Results The ROC curve area for the combination of special staining and immunohistochemical staining was the largest(0.943),indicating the highest diagnostic efficacy.This combined method showed an accuracy of 97.50%,a sensitivity of 98.57%,and a specificity of 90.00%,all of which were significantly higher than those of the individual staining methods(P<0.05).When immunohistochemistry staining was used alone,the accuracy was 92.50%,the sensitivity was 95.71%,and the specificity was 70.00%.The accuracy of Masson staining,PAS staining,and Congo red staining was 82.50%,87.50%and 80.00%respectively,all lower than that of the combined staining method(P<0.05).Conclusion The combination of special staining and immunohistochemistry staining has high application value in tumor diagnosis,significantly improving diagnostic accuracy,sensitivity and specificity,superior to single staining method.This combined technique can provide more reliable evidence for clinical tumor diagnosis.
作者
席乐峰
孙建平
张德臣
王利勤
李凤营
谢福森
XI Lefeng;SUN Jianping;ZHANG Dechen;WANG Liqin;LI Fengying;XIE Fusen(Department of Pathology,Zhengzhou Yihe Hospital,Zhengzhou,Henan 450000,China)
出处
《生物医学工程学进展》
CAS
2024年第3期221-227,共7页
Progress in Biomedical Engineering
关键词
特殊染色
免疫组化染色
肿瘤诊断
诊断效能
ROC曲线
Special Staining
Immunohistochemistry Staining
Tumor Diagnosis
Diagnostic Efficacy
ROC Curve
