摘要
【目的】NAC(NAM, ATAF and CUC)参与植物生长发育和多种逆境胁迫响应过程的调控。本文旨在鉴定和研究对矮牵牛生长发育和逆境胁迫响应的关键NAC成员,为优质抗逆矮牵牛育种提供基因资源。【方法】以腋生矮牵牛(Petunia axillaris)基因组为参考基因组,利用矮牵牛花器官衰老过程、烟草脆裂病毒(Tobacco rattle virus,TRV)侵染、低磷、低温、NaCl、铜离子和干旱胁迫处理后的转录组数据,分析矮牵牛NAC基因(PaNACs)差异表达情况,并对差异表达PaNACs的启动子顺式作用元件及转录因子结合位点进行分析。利用实时荧光定量PCR验证了部分差异表达PaNACs在矮牵牛花衰老过程中的表达情况,并预测了差异表达PaNACs编码蛋白的潜在靶基因。【结果】鉴定的131个PaNAC基因中,59个(45.04%)被鉴定为花器官衰老和逆境胁迫响应过程中的差异表达基因。PaNAC72、 PaNAC22、 PaNAC29、 PaNAC40、 PaNAC2、 PaNAC90、 PaNAC83、 PaNAC56、 PaNAC36和PaNAC35在至少3个生物学过程响应中差异表达显著,其中拟南芥衰老关键基因AtNAP的直系同源基因PaNAC29在花器官衰老过程和低温、低磷、铜离子胁迫逆境处理中显著上调表达;PaNAC72在除受铜离子胁迫外的所有6种处理中表达差异显著;PaNAC22在花器官衰老过程和低温和低磷胁迫中上调表达,在铜离子和干旱逆境下调表达。启动子分析结果显示这10个PaNAC启动子区域存在多种逆境胁迫响应相关元件,且大量响应衰老和逆境胁迫的差异表达基因的启动子区域存在NAC的结合位点。【结论】PaNACs广泛参与矮牵牛生长发育及逆境胁迫响应,其中PaNAC29可能是花衰老关键的正调控因子,PaNAC72广泛响应多种逆境胁迫。
Objective NACs in petunia responsible for the growth,floral senescence,and stress response were identified and analyzed.Method Based on the Petunia axillaris genome,transcriptomes on the flower in senescence as well as some other organs under the stress of inoculated tobacco rattle virus(TRV),low phosphorus,low temperature,NaCl,copper ion,or drought were obtained.Expression under stress,cis-acting elements,and transcription factor binding sites in promoters of differentially expressed PaNACs were analyzed.The expression in flower senescence was determined using qRT-PCR,and putative target genes of proteins encoded by them predicted.Result Of the 131 PaNACs,59(i.e.,45.04%of all)were identified as differentially expressed genes(DEGs)during flower senescence and in response to stresses.PaNAC72,PaNAC22,PaNAC29,PaNAC40,PaNAC2,PaNAC90,PaNAC83,PaNAC56,PaNAC36,and PaNAC35 exhibited significant differential expressions in response to at least 3 stress treatments.Among them,PaNAC29,an orthologue of the Arabidopsis key senescence-related gene AtNAP,was highly upregulated during flower senescence and in response to low temperature,low phosphorus,or copper ion treatment.PaNAC72 was significantly affected by all except copper ion treatment.PaNAC22 was upregulated during flower senescence and in responses to low temperature and low phosphorus treatments but downregulated in the presence of copper ion or under drought condition.Multiple stress responsive elements presented in the promoters of the 10 PaNACs,and many senescence and stress responsive DEGs containing NAC binding sites in their promoters.Conclusion NAC(NAM,ATAF and CUC)involved widely in the growth,development,and stress responses of plants.PaNACs in petunia,such as PaNAC29 appeared to be a key positive regulator of floral senescence,and PaNAC72 responsive to a wide variety of stresses.
作者
杨应杰
张付昆
穆静怡
付鲁峰
陈倬
李华
关夏玉
吕培涛
YANG Yingjie;ZHANG Fukun;MU Jingyi;FU Lufeng;CHEN Zhuo;LI Hua;GUAN Xiayu;LÜPeitao(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2024年第6期700-710,共11页
Fujian Journal of Agricultural Sciences
基金
福建农林大学园艺学院青年学术骨干培养基金项目(722022011)
福建省高原学科建设项目(102/71201801101)。
关键词
矮牵牛
NAC
转录组
衰老
胁迫
生物信息学
Petunia hybrida
NAC
transcriptome
senescence
stress
bioinformatics
